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Barocycler® NEP2320

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The Barocycler NEP3229The compact and versatile Barocycler NEP2320 fills an important need for all laboratories that want improved sample preparation in genomics, proteomics, transcriptomics, and metabolomics. The NEP2320 is a safe and versatile sample preparation instrument designed to use a minimum amount of laboratory bench space, while still providing…the Power of PCT.  The mechanisms of action of the PCT SPS differ significantly from other sample preparation methods.  Specifically, lysis occurs when samples are subjected to alternating cycles of ambient and high pressure, up to 35,000 psi, in the hydrostatic reaction chamber of the Barocycler. The NEP2320 uses available laboratory compressed air, bottled compressed air, or a stand-alone compressor to create high and ultra-high hydrostatic pressure inside the Barocycler reaction chamber. Exquisite control of the PCT process is accomplished by specialized components, a programmable controller, and software.  The NEP2320 quickly and efficiently processes one sample at a time. Although smaller than the NEP3229, the NEP2320 still provides the user with many of the features of the Barocycler NEP3229, including temperature control.

The Barocycler NEP2320 uses the same PULSE Tubes (Pressure Used to Lyse Samples for Extraction) as the NEP3229. PULSE Tubes transmit the Power of PCT from the Barocycler instrument to the sample.  The PULSE Tube is placed in the reaction chamber of the Barocycler NEP2320, submerging it in the chamber fluid, which is distilled water. 

Specifications of the PCT SPS*

Barocycler NEP2320
Maximum Pressure 35 kpsi, 235MPa Protocol Settings Pressure
Holding Time (up or down)
protocol number
Working Pressure Range 5 to 35 kpsi Maximum number of programmable cycles 99
Chamber construction Stainless steel Temp. Range 4 to 37°C
(Temperature control not provided)
Pressure Accuracy +/-2 kpsi Minimum air pressure needed 90psi
Min suggested air compressor capacity / tank size** >2 cfm @ 90psi; >2 gal Number of PULSE Tubes per Chamber 1
Typical Pressurization Time to 35ksi < 5 sec Dimensions 19 × 17 × 19 in
Depressurization Time < 1 sec Weight 80 Ibs
Power Requirement*** 120 VAC, 2 Amps, 60 Hz Laboratory Environment 15 to 30°C, < 85% Humidity

*Specifications may be changed without prior notification
**Faster ramp time is achieved by increasing capacity of air supply
***For US model - can be modified to comply with non-US power requirements

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