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PCT-HD (PCT-SWATH)

PCT-HD (PCT-SWATH)

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Reproducible Tissue Homogenization and Protein Extraction for Quantitative Proteomics using MicroPestle-assisted Pressure Cycling Technology
Reproducible Tissue Homogenization and Protein Extraction for Quantitative Proteomics using MicroPestle-assisted Pressure Cycling Technology
J. Proteome Res., DOI: 10.1021/acs.jproteome.5b01136, Publication Date (Web): April 21, 2016, Copyright © 2016 American Chemical Society


New Australian Cancer Research Facility to Profile 70K Tumor Proteomes Over Next Seven Years - GenomeWeb/Feb 03, 2016 | Adam Bonislawski

Application Note-PCT-HD: Homogenization, Extraction and Digestion of Small Tissue Samples for Proteomic Applications

Minimal sample requirement for highly multiplexed protein quantification in cell lines and tissues by PCT-SWATH mass spectrometry.
Shao S1,2, Guo T2, Koh CC2, Gillessen S3, Joerger M3, Jochum W4, Aebersold R2,5.
1Division of Endocrinology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, P. R. China.
2Department of Biology, Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
3Department of Oncology/Hematology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
4Institute of Pathology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
5Faculty of Science, University of Zurich, Zurich, Switzerland.
Proteomics. 2015 Aug 19. doi: 10.1002/pmic.201500161.


Mass spectrometry-based proteomic quest for diabetes biomarkers
Shiying Shao a,b, Tiannan Guo b, Ruedi Aebersold b,c,
a Division of Endocrinology, Tongji Hospital, Huazhong University of Science & Technology, Wuhan 430030, PR China
b Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Switzerland
c Faculty of Science, University of Zurich, 8057 Zurich, Switzerland
Biochimica et Biophysica Acta 1854 (2015) 519-527


Stratification of Histologically Indistinguishable Prostate Cancer Tissue Biopsy Samples Using Quantitative Proteotypes Obtained by PCT-SWATH
Tiannan Guo1; Li Li2; Ulrich Wagner3; Qing Zhong3, et al.
1Dep Biol,Inst Mol Sys Biol, ETH Zurich, Zurich, Switzerland; 2CECAD, University of Cologne, Cologne, Germany; 3Institute of Surgical Pathology, University Hospit, Zürich, Switzerland; 4Faculty of Science, University of Zurich, Zurich, Switzerland


Rapid mass spectrometric conversion of tissue biopsy samples into permanent quantitative digital proteome maps
Tiannan Guo, Petri Kouvonen, Ching Chiek Koh, Ludovic C Gillet, Witold E Wolski, Hannes L Röst, George Rosenberger, Ben C Collins, Lorenz C Blum, Silke Gillessen, Markus Joerger, Wolfram Jochum & Ruedi Aebersold
Nature Medicine (2015) doi:10.1038/nm.3807


Hands Free Sample Homogenization and Protein Extraction from Small Tissue Biopsy Using Pressure Cycling Technology and MicroPestle
Shiying Shao1,2; Vera Gross3; Wen Yan1,2; Tiannan Guo2; Alexander Lazarev3; Ruedi Aebersold1,4
1Institute of Molecular Systems Biology, ETH, Zurich, Switzerland; 2Huazhong University of Science & Technology,
Wuhan, China; 3Pressure BioSciences, Inc., South Easton, MA; 4University of Zurich, Zurich, Switzerland


More Efficient Tissue Lysis and Protein Digestion with Lower Concentration of Denaturant Using Pressure Cycling Technology
Wen Yan1,2; Shiying Shao1,2; Vera Gross3; Alexander Lazarev3; Tiannan Guo1; Ruedi Aebersold1,4
1Institute of Molecular Systems Biology, ETH, Zurich, Switzerland; 2Huazhong University of Science & Technology,
Wuhan, China; 3Pressure BioSciences, Inc, South Easton, MA; 4University of Zurich, Zurich, Switzerland


Quantifying cancer related proteins via targeting MS assay resources
Ruedi Aebersold, Ph.D


Proteomic investigation of intra-tumor heterogeneity
Tiannan Guo, MD, PhD


Proteotypic features of NCI-60 cancer cells obtained by SWATH-MS predicts phenotypes
Tiannan Guo; Ching Chiek Koh, Michael Menden, Alessandro Ori, Murat Iskar, Wilson Wen Bin, et al.


Analysis of Tissue Biopsies by PCT-SWATH
Tiannan Guo, MD, PhD
Presented at International HUPO 2013
Subset of Slides

Please contact Dr. Tiannan Guo (guo@imsb.biol.ethz.ch) for more information.