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Pressure-Enhanced Enzymology

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PCT-SWATH: pressure cycling technology coupled with SWATH mass spectrometry for fast, deep and reproducible proteomic analysis
Tiannan Guo1, Petri Kouvonen1, Chiek Koh Ching1, Ludovic Gillet1, Witold Wolski1, Hannes Rost1, George Rosenberger1, Silke Gillessen2, Markus Jorger2, Ben Collins1, Alexander Leitner1, Ruedi Aebersold1 1Institute of Molecular Systems Biology, ETH Zurich, Switzerland, 2Department of Internal Medicine, Kantonsspital, Switzerland.

Abstract Only

Please contact Dr. Tiannan Guo (guo@imsb.biol.ethz.ch) for more information.


Analysis of Tissue Biopsies by PCT-SWATH
Tiannan Guo, MD, PhD, Aebersold Lab, IMSB, ETH Zurich Presented at International HUPO 2013

Subset of Slides

Please contact Dr. Tiannan Guo (guo@imsb.biol.ethz.ch) for more information.


Pressure-assisted sample preparation for proteomic analysis
Pawel P. Olszowy a,b, Ariel Burns a, Pawel S. Ciborowski
a Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA
b Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, Poland


Investigation of Pressure-Enhanced Trypsin Proteolysis
Vera Gross; Greta Carlson; Alexander R. Ivanov; Scott Walmsley; Alexey Nesvizhskii; and Alexander Lazarev
Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA, USA ; Barnett Institute, Northeastern University, Boston, MA, USA; University of Michigan, 1301 Catherine St., Ann Arbor, MI


Site specific glycoforms of haptoglobin in liver cirrhosis and hepatocellular carcinoma.
Pompach P, Brnakova Z, Sanda M, Wu J, Edwards N, Goldman R.
Georgetown University, United States.
Mol Cell Proteomics. 2013 Feb 6.


Quantitative LC-MS-MRM analysis of site-specific glycoforms of haptoglobin in liver disease.
Sanda M, Pompach P, Brnakova Z, Wu J, Makambi K, Goldman R.
Georgetown University, United States
Mol Cell Proteomics. 2013 Feb 6.


Rapid Release of N-Linked Glycans from Glycoproteins by Pressure-Cycling Technology
Zoltan Szabo, Andrs Guttman and Barry L. Karger*
Barnett Institute, Northeastern University, Boston, Massachusetts 02115 Anal. Chem., 2010, 82 (6), pp 2588–2593


Mechanistic Investigations of Hydrostatic Pressure Effects on Tryptic Digestion
Alexander Lazarev1; Vera Gross1; Greta Carlson1; Alexander Ivanov2; Scott Walmsley3; Alexey Nesvizhskii3
1 Pressure Bioscience Inc., 14 Norfolk Ave. South Easton, MA 02375 2 Barnett Institute, Northeastern University, 360 Huntington Avenue, Boston MA 02115 3 University of Michigan, 1301 Catherine St., Ann Arbor, MI 48109


Improved proteomic profiling of the cell surface of culture-expanded human bone marrow multipotent stromal cells
Samuel T. Mindayea, Moonjin Raa, Jessica Lo Surdob, Steven Bauerb, and Michail A. Altermana
aTumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD, bCellular and Tissue Therapies Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD
Journal of Proteomics 2012


The Evaluation of Pressure-Assisted Enzymatic Digestion for the Optimal Digestion of Monoclonal Antibodies
Yazen Jmeian; Eric Beil; Dariusz Janecki; Steven Pomerantz; Jennifer Nemeth
Janssen Research & Development, Radnor, PA
Abstract Only

The global proteomic architecture of culture-expanded human bone marrow multipotent stromal cells and the proteomic basis of heterogeneity
Samuel T Mindaye*, Moonjin Ra*, Jessica Lo Surdo**, Steven Bauer**, Michail Alterman*
CBER *Tumor Vaccines and Biotechnology Branch, **Cellular and Tissue Therapies Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research Food

Comparative Proteomics of Swine Tissue 2DLC/MS of Tissue Digestion Preliminary Protein Analysis
Matthew Lawrence
Human Proteomics Program
University of Wisconsin-Madison

Proteolysis-PrEP (Lysozyme): Effect of Pressure Cycling on Lysozyme Activity

Proteomics under Pressure: Development of Essential Sample Preparation Techniques in Proteomics Using Ultrahigh Hydrostatic Pressure
Emily Freeman and Alexander R. Ivanov*
HSPH Proteomics Resource, Department of Genetics and Complex Diseases, Harvard School of Public Health, Harvard University,
655 Huntington Avenue, SPH-1 Room 409, Boston, Massachusetts 02115, United States

Global Deglycosylation in High Throughput LC-MS Glycomics: Comparing Microwave, Pressure, and Ultrasound Effects on PNGase F with Human Serum
Scott R. Kronewitter, Gordon W. Slysz, Daniel López-Ferrer, Matthew E. Monroe, Rui Zhao, Thomas L. Fillmore, Karl K. Weitz, Robert A. Heegel, Daniel J. Orton, Brian L. LaMarche, Kevin L. Crowell, Anuj R. Shah, Carrie D. Nicora, Heather M. Brewer, David G. Camp II, Gordon A. Anderson, Ronald J. Moore, and Richard D. Smith

Development of a 20 kpsi Enzymatic Digester for High Throughput Proteomic Analysis and Its Application to Membrane Proteomics
Seok-Won Hyung1; Daniel López-Ferrer1; Daniel J. Orton1; Erika Zink1; Karl K. Weitz1; Rui Zhao1; Ronald J. Moore1; Kim K. Hixson1; Edmund Y. Ting2; Alexander V. Lazarev2; Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA; 2Pressure Biosciences, Inc, South Easton, MA

MALDI and LCMS Identifications of Early Protein and LA-ICP-MS Identification of Early Metal Biomarkers of Ionizing Radiation
P. Pevsner1, G. Li2, N. Sobol1, D.C Miller1, D. Robertson2,3
1Department of Pathology and Anatomical Sciences
University of Missouri School of Medicine
2 University of Missouri Department of Chemistry
3University of Missouri Research Reactor (MURR)
Columbia, MO, USA

Rapid Protein Extraction and Trypsin Digestion with Pressure Cycling Technology (PCT)
Paul H Pevsner et al., University of Missouri School of Medicine

Improving the Efficiency and Throughput of an Enzymatic Digestion of Klotho using Pressure Cycling Technology (PCT)
Taha Rezai1, Vera Gross2, Alexander Lazarev2, Mary Lopez1
1Thermo Fisher Scientific, Cambridge, MA; 2Pressure BioSciences Inc., Boston, MA
MSACL 2011

Digestion with Pressure Cycling Technology (PCT)
Nicholas Sobol, Jennifer Oprihory*, Tiffany Remsen, A. Yuen, P., Paul Pevsner.
Department of Pathology and Anatomical Sciences
University of Missouri School of Medicine, Columbia, MO

Applications of High Pressure for Automated High Performance Proteomics Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes
Daniel López-Ferrer
Caprion Proteomics US LLC

Recovery and Immunoaffinity Mass Spectrometric Analysis of Integral Membrane Proteins from Metastatic Ovarian Cancer Tissue Using PCT™ and ProteoSolve™-TD Buffers
Dr. Luke V. Schneider, CSO, Target Discovery, Inc.