Pressure-Enhanced Enzymology

Pressurized Pepsin Digestion in Proteomics: An Automatable Alternative to Trypsin for Integrated Top-down Bottom-up Proteomics
Daniel López-Ferrer1*, Konstantinos Petritis1#, Errol W. Robinson2, Kim K. Hixson2, Zhixin, Tian1, Jung Hwa Lee3, Sang-Won Lee3, Nikola Tolic2, Karl K. Weitz1, Mikhail E. Belov1, Richard D. Smith1 and Ljiljana Paša-Tolic2*
1Biological Science Division, 2Environmental Molecular Sciences Laboratory, Pacific Northwest
National Laboratory, Richland, WA, USA and 3Department of Chemistry, Korea University,
Seoul, Korea
A Comparison of Methods for Efficient Digestion of Protein Therapeutics
Lorna L. Maheu, Heather M. Connelly, Adam G. Harder, and Steven L. Cockrill
Analytical Sciences, Amgen Inc., Longmont, CO 80503

Comparison between ultra-high pressure and atmospheric tryptic digestion for proteomic analyses
E. Bonneil1, R. Biringer2, C. Bell1, P. Thibault1
1Institute for Research in Immunology and Cancer Université de Montréal; 2Thermo Fisher Scientific

RAPID SAMPLE PREPARATION METHODS FOR THE ANALYSIS OF N-LINKED GLYCANS
Zoltan Szabo, András Guttman, Tomas Rejtar and Barry L. Karger
Barnett Institute, Boston, MA, USA

Development of Essential Sample Preparation Techniques in Proteomics Using Ultra-High Pressure
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, HSPH Proteomics Resource, Department of Genetics and Complex Diseases, Boston, MA 2115

High Pressure Direct Protein Extraction from Tissue – Trypsin Digest with Pressure Cycling Technology (PCT)
Paul H. Pevsner, Jennifer Oprihory, Nicholas Sobol, Parth Kothiya, Zeineen A. Momin, Naresh Vasani, and Tiffany Remsen
Department of Pathology, University of Missouri School of Medicine, Columbia, MO

Development of new technology approaches for high-throughput proteome analysis
Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes
Daniel López-Ferrer, Biological Separations & Mass Spectrometry Group, Pacific Northwest National Laboratory

Novel efficient alternatives for essential sample preparation techniques in functional proteomics
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, Department of Genetics and Complex Diseases

High-Pressure Assisted In-Gel Tryptic Digestion for Qualitative and Quantitative Characterization of Protein Mixtures
Melkamu Getie-Kebtie and Michael Alterman
FDA-CBER-NIH, Rockville Pike, HFM – 735 Bldg 29A rm 2B20, Bethesda, MD 20892

Tandem Mass Spectrometry Analysis of Ex Vivo Amyloid Fibril and Tissue Samples
Zhenning Hong,1Giuseppe Infusini,1Lawreen H. Connors,2Martha Skinner,2Catherine E. Costello1,2
Mass Spectrometry Resource and 2Amyloid Treatment and Research Program, Boston University School of Medicine, Boston, MA USA

Screening for Drug-Drug Interactions Using a Targeted Proteomics Strategy
Daniel B. Kassel1; Kheng B. Lim1; Melinda Manuel1;
Teruaki Okuda2; Naomi Kamiguchi2; Christie L Hunter3; Brian Williamson3; Lydia Nuwaysir3
1Takeda San Diego, Inc, San Diego, CA; 2Takeda Pharmaceutical Company, Limited, Osaka, Japan;
3AB Sciex, Foster City, CA

Application of High Pressure and Highly Stable Trypsin Aggregate Coating on Superparamagnetic Magnetite/Silica Nanoparticles for High Performance Proteomics
Daniel López-Ferrer1, Byoungsoo Lee2, Jungbae Kim2 and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA 99352; 2Korea University, Seoul, Korea

A separation-free quantitative MS-based profiling approach using 2-AA isotopically labeled substrates for high-throughput glycan screening
Justin M. Prien1, Lorna Maheu1, Brad Prater1, and Steve Cockrill1
1Analytical Science, Amgen, Longmont, Colorado 80503

New Strategies for High Pressure-Assisted Digestion in Proteomics
Kim K. Hixson1,2, Daniel López-Ferrer1, Karl K. Weitz1, Ronald J. Moore1, Scott R. Kronewitter1, Heather H. Smallwood3, Mikhail E. Belov1, and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA; 2Washington State University, Pullman, WA; St. Jude Children’s Research Hospital, Memphis, TN

Rapid Release of N-Linked Glycans from Glycoproteins by Pressure-Cycling Technology
Zoltan Szabo, Andras Guttman and Barry L. Karger
Barnett Institute, Northeastern University, Boston

High Throughput Pressure-Enhanced Protein Extraction and Enzymatic Digestion With Pressure Cycling Technology (PCT) and PCT MicroTubes
C. Dussault, G. Carlson, V. Gross, N. Lawrence, A. Lazarev, M. Potter, R. T. Schumacher, and E. Ting

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Roger Biringer1, Eric Bonneil2, Julian Saba1, Andreas Huhmer1, Pierre Thibault2

A Comparative Study of In-gel Digestions Using Microwave and Pressure Accelerated Technologies
Alvarado, Diana Tran, Bonnie Ching and Brett S. Phinney
University of California Davis

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Roger Biringer1, Eric Bonneil2, Julian Saba1, Andreas Huhmer1, Pierre Thibault2
1Thermo Fisher Scientific, San Jose, CA 2Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Canada
ABRF 2010

Label-free mass spectrometry-based relative quantitation of proteins separated by 1DE
Melkamu Getie-Kebtie1; Maryna Eichelberger2, Manju B. Joshi13, Rajesh Gupta3, Michail A. Alterman1
1FDA/CBER/OCTGT/TVBB; 2FDA/CBER/OVRR/LPRVD; 3FDA/CBER/OVRR/PQLS

Application of mass spectrometry-based proteomics in influenza virus research and vaccine manufacturing
Melkamu Getie-Kebtie¹; Maryna Eichelberger²; Michail Alterman¹
1FDA/CBER/OCTGT/TVBB, Bethesda, MD; 2FDA/CBER/OVRR/DVP, Bethesda, MD
US HUPO 2010

Novel efficient alternatives for sample preparation techniques crucial for functional proteomics
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, Department of Genetics and Complex Diseases
US HUPO 2010

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Eric Bonneil¹; Roger Biringer²; Julian Saba²; Andreas Huhmer²; Pierre Thibault¹
Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Canada
Thermo Fisher Scientific, San Jose, CA. US HUPO 2010

Rapid Release of N-Linked Glycans from Glycoproteins by Pressure-Cycling Technology
Zoltan Szabo, Andras Guttman, and Barry L. Karger*
Barnett Institute, Northeastern University, Boston, Massachusetts 02115

Pressure-Enhanced Enzymatic Proteolysis
J. Behnke, G. Carlson, J. Damasio, C. Dussault, W. Fritzsche, V. Gross, J. Lanuza, N. Lawrence, A. Lazarev, L. Manning, R. McCloskey, M. Potter, C. Saravis, R. T. Schumacher, G. Smejkal, M. Thomson, and E. Ting

Proteomics Under Pressure: Rapid Extraction and Digestion in a Single Tube.
Alexander V. Lazarev¹; Emily Freeman²; Vera S. Gross¹; Greta Carlson¹; Edmund Ting¹; Alexander R. Ivanov²Pressure BioSciences, South Easton, MA ; 2Harvard School of Public Health, Boston, MA

High pressure-assisted in-gel tryptic digestion: qualitative and quantitative aspects
Michail Alterman¹; Melkamu Getie-Kebtie¹; Alexander Lazarev²; Vera S. Gross²
1FDA/CBER/OCTGT/TVBB, Bethesda, MD; 2Pressure BioSciences, Inc, South Easton, MA

Ultra-Rapid Pressure Digestion and Label-Free Quantitative Proteomics of Yersiniae Infected Mice Tissues
Kim K. Hixson¹, Daniel López-Ferrer¹, Matthew Bender², Patricia L. Worsham³, Karl K. Weitz¹, Nate Lawrence⁴, Amy Rasley⁵, Therese W. Clauss¹, Ljiljana Pasa-Tolió¹, Richard D. Smith¹, Mary S. Lipton¹
Pacific Northwest National Laboratory, Richland WA; ²NBACC, Washington, DC; ³USAMRIID, Frederick, MD; ⁴Pressure BioSciences, Inc., South Easton, MA; ⁵Lawrence Livermore National Lab, Livermore, CA

Improved Protein Coverage and Throughput in Proteomics using On-Line Multiplexed Enzyme Digestions and Targeted MS/MS with a Modified LTQ-FTICR
Daniel López-Ferrer¹, Konstantinos Petritis¹, Andrey Liyu¹,Jung Hwa Lee², Sang-Won Lee², Benito Cañas³, Kim K. Hixson¹, Richard D. Smith¹ and Mikhail E. Belov¹ 1Pacific Northwest National Laboratory, Richland, WA; Korea University, ²Seoul, Korea; ³Universidad Complutense de Madrid, Madrid ASMS 2009

High Pressure Trypsin Digestion of Proteins for Proteomic Analysis
Shane A. Wyatt; TIMOTHY R. CROLEY Commonwealth of Virginia, Richmond, VA

A Comparison of Methods for Efficient Digestion of Protein Therapeutics
Lorna L. Maheu, Heather M. Connelly, Adam G. Harder, and Steven L. Cockrill Analytical Sciences, Amgen Inc., Longmont, CO 80503

Application of Pressurized Solvents for Ultrafast Trypsin Hydrolysis in Proteomics: Proteomics on the Fly
Daniel López-Ferrer, Konstantinos Petritis, Kim K. Hixson, Tyler H Heibeck, Ronald J. Moore; Mikhail E. Belov, David G. Camp, II, and Richard D. Smith

IMAGING MALDI of Colorectal Carcinoma - Field Defects in SatelliteTissue
Tiffany Remsen , M Momeni, P Kessler , F Francois , A Stern , S. Anand and P. Pevsner

Unified Sample Preparation Approach Using Hydrostatic Pressure Cycling: Simultaneous Isolation of Proteins, Nucleic Acids and Lipids from a Single Sample
Vera S. Gross; Greta Carlson; Gary B. Smejkal; Ada T. Kwan; Timothy Straub; Alexander V. Lazarev (2008)

Application of Pressurized Solvents for Ultra-Fast Proteolysis: Proteomics on the Fly
Daniel López-Ferrer, Konstantinos Petritis, Natacha M. Lourette, Brian Clowers, Kim K. Hixson, Tyler Heibeck, Eric Livesay, Ryan Kelly, David C Prior, Ljiljana Pasa-Tolic, Mikhail E. Belov, David G. Camp II, and Richard D. Smith. Pacific Northwest National Laboratory, Richland, WA