Publications

1997-2009 PBI Publication and Posters Archive

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Simple Protocols for Isolation of Intact Mitochondria Using The SHREDDER SG3 and PBI Mitochondria Isolation Kits
Pressure BioSciences, South Easton, MA 02375


• Optimization of high pressure-assisted methods for proteomic sample preparation
  Alexander V. Lazarev1*; Vera S. Gross1; Greta Carlson1; Edmund Ting1; Emily Freeman2;
  Alexander R. Ivanov2 ; Melkamu Getie-Kebtie3; Michail Alterman3
  1Pressure BioSciences, South Easton, MA, USA; 2Harvard School of Public Health, Boston, MA, USA; 3FDA, CBER
  Rockville , MD, USA
  


• Pressure Cycling for Sample Preparation
  PCT System Provides Automated Alternative to Manual Methods
  Richard T. Schumacher, Vera Gross, Ph.D., Edmund Y. Ting, ScD., and Alexander Lazarev, Ph.D
• Complete Disruption of Caenorhabditis elegans Under Non-denaturing Conditions Using Pressure Cycling Technology (PCT)
  
• Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
  Vera Gross, Greta Carlson, Ada T Kwan, Gary Smejkal, Emily Freeman, Alexander R Ivanov, Alexander Lazarev
  
• Isolation of Mitochondria from Cell Cultures by PCT for Proteomic Analysis
  Vera Gross, Alexander V. Lazarev, Nathan P. Lawrence, and Richard T. Schumacher
  Pressure BioSciences, Inc., South Easton, MA
  
• Smejkal & Schweitzer ERP article published: "Will current technologies enable dinosaur proteomics?
  Gary B. Smejkal, Mary H. Schweitzer
  Future Drugs, Ltd. 2007
  
• Solution phase isoelectric fractionation in the multicompartment electrolyser: A divide and conquer strategy for the analysis of complex proteomes
  Gary B. Smejkal, Alexander Lazarev
  BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. VOL 4. NO 1. 76–81. MAY 2005
  
• Pressure Cycling Technology (PCT) Applications in Extraction of Biomolecules from Challenging Biological Sample
  Feng Tao, Chunqin Li, Gary Smejkal, Alexander Lazarev, Nathan Lawrence, and Richard T. Schumacher
  Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology , Vol. 1, 166-173, 2007
  
• Proteomic Analyses of Proteins Isolated From Mammalian Bone
  Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier
  HUPO 6th Annual World Congress Seoul, October 6 – 10, 2007
  
• "Pressure Cycling Technology (PCT): a Novel Sample Preparation Approach to Biomarker Discovery and Drug Development in Lipid-Rich Samples"
  Ilyana Romanovsky, Gary Smejkal, Alexander Lazarev, Nate Lawrence, and Richard T. Schumacher
  Next Generation Pharmaceutical
  
• Application of pressure cycling technology to tissue sample preparation for 2-DE
  Heather Ringham, Richard L. Bell, Gary B. Smejkal, James Behnke, Frank A. Witzmann
  ELECTROPHORESIS, March 6 2007, Volume 28, Issue 6, Pages: 1022-1024
  
  
Pressure Cycling for Sample Preparation
PCT System Provides Automated Alternative to Manual Methods
Richard T. Schumacher, Vera Gross, Ph.D., Edmund Y. Ting, ScD., and Alexander Lazarev, Ph.D

Complete Disruption of Caenorhabditis elegans Under Non-denaturing Conditions Using Pressure Cycling Technology (PCT)


Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
Vera Gross, Greta Carlson, Ada T Kwan, Gary Smejkal, Emily Freeman, Alexander R Ivanov, Alexander Lazarev


Isolation of Mitochondria from Cell Cultures by PCT for Proteomic Analysis
Vera Gross, Alexander V. Lazarev, Nathan P. Lawrence, and Richard T. Schumacher
Pressure BioSciences, Inc., South Easton, MA


Smejkal & Schweitzer ERP article published: "Will current technologies enable dinosaur proteomics?
Gary B. Smejkal, Mary H. Schweitzer
Future Drugs, Ltd. 2007


Solution phase isoelectric fractionation in the multicompartment electrolyser: A divide and conquer strategy for the analysis of complex proteomes
Gary B. Smejkal, Alexander Lazarev
BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. VOL 4. NO 1. 76–81. MAY 2005


Pressure Cycling Technology (PCT) Applications in Extraction of Biomolecules from Challenging Biological Sample
Feng Tao, Chunqin Li, Gary Smejkal, Alexander Lazarev, Nathan Lawrence, and Richard T. Schumacher
Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology , Vol. 1, 166-173, 2007


Proteomic Analyses of Proteins Isolated From Mammalian Bone
Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier
HUPO 6th Annual World Congress Seoul, October 6 – 10, 2007


"Pressure Cycling Technology (PCT): a Novel Sample Preparation Approach to Biomarker Discovery and Drug Development in Lipid-Rich Samples"
Ilyana Romanovsky, Gary Smejkal, Alexander Lazarev, Nate Lawrence, and Richard T. Schumacher
Next Generation Pharmaceutical


Tris interference in IEF and 2-DE
Gary B. Smejkal, Myra H. Robinson
ELECTROPHORESIS May 10 2007, Volume 28, Issue 10, Pages: 1601-1606

Application of pressure cycling technology to tissue sample preparation for 2-DE
Heather Ringham, Richard L. Bell, Gary B. Smejkal, James Behnke, Frank A. Witzmann
ELECTROPHORESIS, March 6 2007, Volume 28, Issue 6, Pages: 1022-1024


• Sample Preparation for Two-Dimensional Gel Electrophoresis using Pressure Cycling Technology
  Gary B. Smejkal, Frank A. Witzmann, Heather Ringham, Deena Small, Susan F. Chase, James Behnke and Edmund Ting
  Smejkal G.B. (2006). I'm an - Omics, You're an -Omics. Expert Rev. Proteomics, 3, 383-385.
  
• "Proteome Society Meeting".
  Tao, F. and Smejkal, G.
  Expert Rev. Proteomics, (2006), 3(1), 7-8
  
• "The promise of gel-based proteomics"
  Smejkal G.B.
  Expert Opinion in Drug Discovery, (2006), 1, 7-10.
  
• "Applications of Pressure Cycling Technology (PCT) in Proteomics"
  Feng Tao, Behnke, J., Li, C., Saravis, C., Schumacher, R.T., and Lawrence, N.P.
  Separation Methods in Proteomics, (2006), Smejkal, G.B. and Lazarev, A., Eds., CRC Press, Taylor & Francis, Baton Rouge, pp. 3 -18.
  
• "Simultaneous Reduction and Alkylation of Protein Disulfides in a Centrifugal Ultrafiltration Device Prior to Two-Dimensional Gel Electrophoresis"
  Gary B. Smejkal, Li C., Robinson M.H., Lazarev, A.V., Lawrence, N.P., and Chernokalskaya, E.
  J. Proteome Res., (2006), 5 (4), 983-987.
  
• "Increased Protein Yields from Escherichia coli Using Pressure Cycling Technology"
  Gary B. Smejkal, Robinson M.H., Lawrence, N.P., Tao, F., Saravis, C.A., and Schumacher, R.T.
  J. of Biomolecular Techniques, (2006), 17:173-175.
  
• "Biological Sample Preparation System Using Pressure Cycling Technology (PCT)"
  Tao, F, Lawrence, N.P., Miller, W.W., Li, C., Tuzmen, P., Behnke, J., Nakhai, B., Kakita, A., Christian, T., Reed, D. Manak, M.M., Schumacher, R.T.
  Advances in High Pressure Bioscience and Biotechnology II, (2003), Springer, New York, p413-417
  
• "Tired of the Same Old Grind in the New Genomics and Proteomics Era?"
  Garret, PE, Tao, F, Lawrence, N, Ji, J, Schumacher, RT, Manak, M
  TARGETS, (2002), 1 (5): 156-162
  
• "Automated Solution for Sample Preparation: Nucleic Acid and Protein Extraction From Cells and Tissues Using Pressure Cycling Technology (PCT)"
  Schumacher, RT, Manak, M, Garrett, P, Miller W, Lawrence, N, Tao, F
  Am. Laboratory, (2002), 34(16): 38-43
  
• "Inactivation of viruses in plasma by cycled pulses of high pressure."
  Dusing, S., Li, C., Behnke, J., Manak, M. and Schumacher, R.
  Trends in High Pressure Bioscience and Biotechnology, (2001), R. Hayashi, ed. Elsevier Science B.V. 355-359

• "Pre-analytical sterilization of serum by pressure cycling treatment:
  A novel procedure to prevent laboratory acquired infections."
  Remaley, A., Hess, R., Fischer, S., Sampson, M. and Manak, M.
  Clin Chem 46:, (2000), (S6) A39.

• "Pressure Cycling Technology: A Novel Approach to Viral Inactivation in Plasma."
  Bradley, D.W., Hess, R.A., Tao, F., Sciaba-Lentz, L., Remaley, A.T., Laugharn, J. A., Manak, M.
  Transfusion, (2000), 40, 193-200.
• "A bioseparation apparatus with high-pressure fluid injection and fluid sampling."
  Dreier G.H., Tao F., Hess R.A., Cheung C.Y., Sciaba L.E., Green D.G., Laugharn J.A. Jr.
  Analytical Biochemistry, (1999), 269(2), 223-9.
• "Unusual Properties of Highly Charged Buffers: Large Ionization Volumes
  and Low Barrier Hydrogen Bonds."
  Hess, R.A., Reinhard, L.A.
  JACS, (1999), 121 (42), 9867-9870.
• "Sample preparation for molecular diagnostics by pressure cycling."
  Laugharn J.A.Jr., Tao F., Sciaba-Lentz L., Bradley, D.W., Hess R.A.
  High Pressure Bioscience & Biotechnology, (1998), Ludwig (Ed.), Springer, New York.
• "Use of High Pressure to Accelerate Antibody:Antigen Binding Kinetics Demonstrated in
  and HIV-1 p24:Anti-HIV-1 p24 Assay."
  Green, D.J, Litt, G.J., and Laugharn, J.A. Jr.
  Clin. Chem., (1998), 44, 341-342.
• "High-pressure-mediated dissociation of immune complexes demonstrated in model systems."
  Cheung, C.Y., Green, D.J., Litt, G.J. and Laugharn, J.A., Jr.
  Clin. Chem., (1998), 44, 299-303.
• "Reversible inhibition of lambda exonuclease with high pressure."
  Rudd, E.A.
  Biochem. Biophys. Res. Commun, (1997), 230, 140-142.

Posters

• Alexander V. Lazarev1; Emily Freeman2; Vera S. Gross1; Greta Carlson1; Edmund Ting1; Alexander R. Ivanov2
  1Pressure BioSciences, South Easton, MA ; 2Harvard School of Public Health, Boston, MA
  Proteomics Under Pressure: Rapid Extraction and Digestion in a Single Tube
  


• R. T. Schumacher, M. Potter, A. Lazarev, E. Ting, and N. Lawrence
  Multiplex Biomolecule Extraction Using the Pressure Cycling Technology (PCT) Sample Preparation System (PCT SPS)
• Vera Gross¹; Irina Stavrovskaya²; Sergei Baranov²; Greta Carlson¹; Emily Freeman³; Alexander Ivanov³; Bruce Kristal²; Alexander Lazarev^{1
  1}Pressure BioSciences, Inc, South Easton, MA; ²Brigham and Women's Hospital; Harvard University, Boston, MA; ³Harvard School of Public Health, Boston, MA US HUPO 2010
  Improved protocols for isolation of intact mitochondria from tissue samples
• Richard T. Schumacher, G. Smejkal, L. Connell, V. Gross, G. Carlson, C. Dussault, E. Ting, M. Potter, J. Lanuza, A. Lazarev, and N. Lawrence (2009)
  Improved Extraction of Biomolecules from Plants, Animals, and Fungi with The PCT Shedder
• Human Proteome Organization (HUPO): HUPO 8th Annual World Congress (2009)
  An Integrated Sample Preparation Workflow Provides Results in Hours: Combining Pressure Cycling Technology (PCT®) and Parallel Isoelectric Focusing (dPC®)
• Vera S. Gross Greta Carlson Alexander V. Lazarev Emily Freeman, Alexander R. Ivanov (2009)
  Optimization of Rapid Protein Digestion Using Pressure Cycling Technology
• Gary B. Smejkal, W. Kelley Thomas, Darren Bauer, Michael Kinter, Ada Kwan, Frank Witzmann, and Heather Ringham (2007)
  "Proteomic analysis of individual Daphnia microcrustaceans"
  
• G.B. Smejkal, J. Anderson, W.K. Thomas, M.H. Robinson, A.T. Kwan, G. Carlson, V. Gross, R.T. Schumacher and A.V. Lazarev (2008)
  Pressure Cycling Technology (PCT) mediated sample preparation schemes for isolating proteins from the nematode Caenorhabditis elegans
• Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, Sumithra Urs, and Deena Small (2008)
  "Isolation of Proteins from Small Subcutaneous Murine Tumor and Adipose Tissues Using a Pressure Cycling Technology"
• Vera Gross, Greta Carlson, Gary Smejkal, Ada Kwan, Emily Freeman,Alexander R. Ivanov and Alexander Lazarev (2008)
  "Tissue Fractionation by Hydrostatic Pressure Cycling: The Unified Sample Preparation Technique for Systems Biology Studies"
• Gary Smejkal, AdaKwan, Michael Zianni, David Mandich, Mary Mason, and Jennifer Koch (2008)
  "Pressure Cycling Technology (PCT) mediated sample preparation Pressure Cycling Technology (PCT) mediated sample preparation schemes enabling high quality two-dimensional gel electrophoresis of Fagus grandifolia tree bark proteins"
Pressure Cycling Technology (PCT) mediated sample preparation schemes for isolating proteins from the nematode Caenorhabditis elegans
G.B. Smejkal, J. Anderson, W.K. Thomas, M.H. Robinson, A.T. Kwan, G. Carlson, V. Gross, R.T. Schumacher and A.V. Lazarev (2008)

"Isolation of Proteins from Small Subcutaneous Murine Tumor and Adipose Tissues Using a Pressure Cycling Technology"
Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, Sumithra Urs, and Deena Small (2008)

"Tissue Fractionation by Hydrostatic Pressure Cycling: The Unified Sample Preparation Technique for Systems Biology Studies"
Vera Gross, Greta Carlson, Gary Smejkal, Ada Kwan, Emily Freeman,Alexander R. Ivanov and Alexander Lazarev (2008)

"Pressure Cycling Technology (PCT) mediated sample preparation Pressure Cycling Technology (PCT) mediated sample preparation schemes enabling high quality two-dimensional gel electrophoresis of Fagus grandifolia tree bark proteins"
Gary Smejkal, AdaKwan, Michael Zianni, David Mandich, Mary Mason, and Jennifer Koch (2008)

Gary Smejkal, Ada Kwan, Vera Gross, Alexander Lazarev, Kelley Thomas, Darren Bauer, Richard Clements, Cesario Bianchi, Frank Sellke, Michael Zianni, David Mandich, Mary Morgan, and Jennifer Koch (2007)
"The Evolution of Sample Preparation in Proteomics: Applications of Pressure Cycling Technology (PCT) Spanning from Bacteria to Homo sapiens"

Gary B. Smejkal, W. Kelley Thomas, Darren Bauer, Michael Kinter, Ada Kwan, Frank Witzmann, and Heather Ringham (2007)
"Proteomic analysis of individual Daphnia microcrustaceans"


Alexander Lazarev, Gary Smejkal, Ilyana Romanovsky, Haiming Cao, Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Adipose Tissue Using Detergent-Free Protein Extraction by Pressure Cycling and High Resolution Tandem Mass Spectrometry"


Gary B. Smejkal, Ada T. Kwan, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier (2007)
"Electrophoretic Analyses of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling Technology"
-9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry, Cambridge, MA

Alexander Lazarev; Gary Smejkal; Ilyana Romanovsky; Ada Kwan; Haiming Cao; Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Murine Adipose Tissue Using Pressure Cycling Technology and High Resolution Tandem Mass Spectrometry U.S. HUPO (2007)"

Gary B. Smejkal, D. C. Muddiman, M. H. Schweitzer, T. S. Collier and A. T. Kwan (2007)
"Electrophoretic Analyses of Proteins and Peptides Isolated From Cortical Bone Using a Pressure Cycling Technology"
-Third Annual US HUPO Conference, Seattle WA.


• Gary Smejkal, Ada Kwan, Vera Gross, Alexander Lazarev, Kelley Thomas, Darren Bauer, Richard Clements, Cesario Bianchi, Frank Sellke, Michael Zianni, David Mandich, Mary Morgan, and Jennifer Koch (2007)
  "The Evolution of Sample Preparation in Proteomics: Applications of Pressure Cycling Technology (PCT) Spanning from Bacteria to Homo sapiens"
• Alexander Lazarev, Gary Smejkal, Ilyana Romanovsky, Haiming Cao, Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
  "Proteomic Analysis of Adipose Tissue Using Detergent-Free Protein Extraction by Pressure Cycling and High Resolution Tandem Mass Spectrometry"
  
• Gary B. Smejkal, Ada T. Kwan, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier (2007)
  "Electrophoretic Analyses of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling Technology"
  -9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry, Cambridge, MA
• Alexander Lazarev; Gary Smejkal; Ilyana Romanovsky; Ada Kwan; Haiming Cao; Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
  "Proteomic Analysis of Murine Adipose Tissue Using Pressure Cycling Technology and High Resolution Tandem Mass Spectrometry U.S. HUPO (2007)"
• Gary B. Smejkal, D. C. Muddiman, M. H. Schweitzer, T. S. Collier and A. T. Kwan (2007)
  "Electrophoretic Analyses of Proteins and Peptides Isolated From Cortical Bone Using a Pressure Cycling Technology"
  -Third Annual US HUPO Conference, Seattle WA.
• G. B. Smejkal, F. Witzmann, H. Ringham, D. Small, E. Ting, I. Romanovsky, and A. Lazarev (2006).
  "Applications of Pressure Cycling Technology (PCT) to Sample Preparation for Proteomic Analyses by Two-Dimensional Gel Electrophoresis"
  -HUPO 5th International World Congress, Long Beach, CA
• Gary Smejkal, Witzmann, F., Ringham, H., Small, D., Chase, S., Thomas, W.K., Morris, K., Abebe, E., and Behnke, J. (2006).
  "Applications of Pressure Cycling Technology (PCT) to Sample Preparation for Two-Dimensional Gel Electrophoresis".
  - 7th Siena Meeting: From Genome to Proteome, Back to the Future. Siena, Italy. September 3-7, 2006.
• Feng Tao, C. Li, A. Lazarev, N. Lawrence, and R.T. Schumacher (2006)
  "Extraction of Nucleic Acids from Challenging Biological Samples Using Pressure Cycling Technology"
  - 2006 CHI, Nucleic Acid-Based Technologies-Meeting the Demands, Arlington, VA
• Gary Smejkal, D. Himali, A. Hanneman, and V. Reinhold (2006)
  "Breaking the Tough Cuticle of Caenorhabditis elegans Using Pressure Cycling Technology and Optimized Lysis Reagents: Giving the Worms a Perm"
  - 2006 US HUPO Second Annual Conference, Boston, MA
• Gary Smejkal, M. Robinson, A. Lazarev, C. Li, J. Behnke, F. Tao, R.T. Schumacher, and N. Lawrence (2006)
  "Application of Pressure Cycling Technology (PCT) in Proteomics: Increased Cell Lysis and Protein Yields from Model Organisms"
  - 2006 US HUPO Second Annual Conference, Boston, MA
• Gary Smejkal, A. Lazarev, F. Tao, N. Lawrence, and R.T. Schumacher (2006)
  "A Comparison of Pressure Cycling Technology (PCT) to Other Methods for Protein Extraction"
  - 2006 MASSEP Eighth Symposium, North Eastern University, Boston, MA
• Smejkal, G., F. Tao, N. Lawrence, and R.T. Schumacher (2006)
  "A Comparison of Pressure Cycling Technology (PCT) to Other Methods of Protein Extraction"
  - 2006 AACC, Oakridge Conference, San Jose, CA
• Harrington, S., McCouch, S., Tao, F., Lawrence, N., Schumacher, R.T. (2004)
  "Use of Pressure Cycling Technology (PCT) for the Release of DNA from Plants"
  - Annual Plant, Animal and Microbes Genomics Conference, San Diego, CA
• Tao, F., Lawrence, N., Li, C., Behnke, J., Lathey, J., Manak, M., & Schumacher, R.T. (2004)
  "Deciphering Mechanisms for Infectious Agent Inactivation Using Cyclic High Pressure or Pressure Cycling Technology (PCT)"
  - Annual Biophysical Society Meeting, Baltimore, MD
• Lawrence, N., Tao, F., Kakita, A., Christian, T., Petersen, D., Behnke, J., Li, C., Saravis, C., Manak, M, & Schumacher, R.T. (2004)
  "Introduction of a Bench Top Instrument Employing Pressure Cycling Technology (PCT) for the Extraction of Nucleic Acids and Proteins"
  - PITTCON2004, Chicago, IL
• Tao, F., Feng, ZC, Lawrence, NP, & Schumacher, R.T. (2004)
  "Deciphering the Mechanisms of Action in the Release of Molecules from Biological Samples Using Pressure cycling Technology (PCT)"
  - Poster at 10th Annual FDA Science Forum, Washington, DC
• Lathey, J.L., Nakhai, B., Tuzmen, P., Tao, F., Schumacher, R.T., Manak, M.M, & Lawrence, N.P. (2004)
  "Inactivation of HIV Inactivation of HIV-1 using Pressure Cycling Technology (PCT)"
  - ASM Conference, New Orleans, LA
• Tao, F., Lawrence, N.P., Behnke, J., Li, C., Manak, M., Saravis, C. & Schumacher, R. (2004)
  "Biological Sample Extraction Using Pressure Cycling Technology (PCT)"
  - Poster at 6th National NIH SBIR Conference, Bethesda, MD
• Schumacher, R., Behnke, J., Li, C., Lawrence, N.P. & Tao, F. (2004)
  "A New Benchtop System for Sample Preparation Using Pressure Cycling Technology (PCT)"
  - Poster at the GSAC XVI, Washington, DC