Scientific Meetings

Harvard Symposium

Symposium: Agenda and Abstracts: Applications of Ultra-High Pressure in Biotechnology
Harvard Medical School Joseph B. Martin Conference Center

VIDEO: Symposium: Application of Ultra-high Pressure in Biotechnology.

Slide Presentations

(Fundamentals of) High-pressure Instruments for Innovation and Discovery
Edmund Ting, Sc.D., Pressure BioSciences

Development of Essential Sample Preparation Techniques in Proteomics Using Ultra-High Pressure
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, HSPH Proteomics Resource, Department of Genetics and Complex Diseases, Boston, MA 2115

Comparison between ultra-high pressure and atmospheric tryptic digestion for proteomic analyses
E. Bonneil1, R. Biringer2, C. Bell1, P. Thibault1
1Institute for Research in Immunology and Cancer Université de Montréal; 2Thermo Fisher Scientific

Development of new technology approaches for high-throughput proteome analysis
Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes
Daniel López-Ferrer, Biological Separations & Mass Spectrometry Group, Pacific Northwest National Laboratory

High Pressure Direct Protein Extraction from Tissue – Trypsin Digest with Pressure Cycling Technology (PCT)
Paul H. Pevsner, Jennifer Oprihory, Nicholas Sobol, Parth Kothiya, Zeineen A. Momin, Naresh Vasani, and Tiffany Remsen
Department of Pathology, University of Missouri School of Medicine, Columbia, MO

Membrane Protein Extraction and Biomarker Analysis from Solid Metastatic Ovarian Tumors with ProteoSolve™-TD Buffers and Pressure Cycling Technology
Dr. Luke V. Schneider, CSO, Target Discovery, Inc.

A Need for Improved Sample Inactivation and Extraction Methods to Support Systems Biology Analysis of Biological Threat Organisms
1 Bradford S. Powell and 2 Loreen L. Lofts
1 Bacteriology Division, 2 Virology Division
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702

Convenient and Reliable Extraction of Borrelia burgdorferi from Ticks Using Pressure Cycling Technology and the PCT Shredder
Richard C. Tilton, Ph.D.
Medical Diagnostics Laboratories

Pressure Cycling Technology (PCT) Augments Sensitivity of Detection and Robustness in Forensic DNA Analyses
Bruce Budowle
Department of Forensic and Investigative Genetics, Institute of Investigative Genetics, University of North Texas Health Science Center, Ft. Worth, Texas

RAPID SAMPLE PREPARATION METHODS FOR THE ANALYSIS OF N-LINKED GLYCANS
Zoltan Szabo, András Guttman, Tomas Rejtar and Barry L. Karger
Barnett Institute, Boston, MA, USA

High-Pressure Assisted In-Gel Tryptic Digestion for Qualitative and Quantitative Characterization of Protein Mixtures
Melkamu Getie-Kebtie and Michael Alterman
FDA-CBER-NIH, Rockville Pike, HFM – 735 Bldg 29A rm 2B20, Bethesda, MD 20892

High Pressure in Life Sciences – Trends and Future Opportunities
Alexander Lazarev, PhD, Pressure BioSciences, Inc.

High Pressure Bioscience and Biotechnology (HPBB2010)

Optimization of high pressure-assisted methods for proteomic sample preparation
Alexander V. Lazarev1*; Vera S. Gross1; Greta Carlson1; Edmund Ting1; Emily Freeman2;
Alexander R. Ivanov2 ; Melkamu Getie-Kebtie3; Michail Alterman3
1Pressure BioSciences, South Easton, MA, USA; 2Harvard School of Public Health, Boston, MA, USA; 3FDA, CBER
Rockville , MD, USA

APS 2010

Improved Extraction of DNA of Ca. Liberibacter Species from Plants and Cultivated Cells Using Pressure Cycling Technology
A. Sechler1, A Marques2, N. Lawrence3, N. W. Schaad1
1FDWSRU, USDA-ARS, Fort Detrick, MD USA
2EMBRAPA, Brasilia, Brasil
3Pressure BioSciences Inc., South Easton, MA, USA

Neuronal Development, Synaptic Function & Behavior C. elegans 2010

Pressure mediated scheme for isolating native proteins from Caenorhabditis elegans under mild non-denaturing conditions
Gary B. Smejkal1,2, Thomas E. Strader3, W. Kelley Thomas1, Jobriah Anderson1, Krystalynne Morris1, and Winston P. Kuo2
1 Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH. 2 Harvard Medical School, Harvard Catalyst, Laboratory for Innovative Translational Technologies, Boston, MA. 3 Pressure BioSciences, South Easton, MA

ASMS 2010

Novel efficient alternatives for essential sample preparation techniques in functional proteomics
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, Department of Genetics and Complex Diseases

Tandem Mass Spectrometry Analysis of Ex Vivo Amyloid Fibril and Tissue Samples
Zhenning Hong,1Giuseppe Infusini,1Lawreen H. Connors,2Martha Skinner,2Catherine E. Costello1,2
Mass Spectrometry Resource and 2Amyloid Treatment and Research Program, Boston University School of Medicine, Boston, MA USA

Proteomic Comparison of Sun-protected vs -Exposed and Young vs Gently-Aged Human Epithelium
1 Facility Center California R.A. Eigenheer1, J.T. Smilowitz2 and B.S. Phinney1
1. Proteomics Core Facility, Genome Center, University of California, Davis
2. Department of Food Science and Technology, University of California, Davis

High Throughput Pressure-Enhanced Protein Extraction and Enzymatic Digestion With Pressure Cycling Technology (PCT) and PCT MicroTubes
C. Dussault, G. Carlson, V. Gross, N. Lawrence, A. Lazarev, M. Potter, R. T. Schumacher, and E. Ting
Pressure BioSciences, Inc. 14 Norfolk Avenue, S. Easton, MA 02375

Screening for Drug-Drug Interactions Using a Targeted Proteomics Strategy
Daniel B. Kassel1; Kheng B. Lim1; Melinda Manuel1;
Teruaki Okuda2; Naomi Kamiguchi2; Christie L Hunter3; Brian Williamson3; Lydia Nuwaysir3
1Takeda San Diego, Inc, San Diego, CA; 2Takeda Pharmaceutical Company, Limited, Osaka, Japan;
3AB Sciex, Foster City, CA

Application of High Pressure and Highly Stable Trypsin Aggregate Coating on Superparamagnetic Magnetite/Silica Nanoparticles for High Performance Proteomics
Daniel López-Ferrer1, Byoungsoo Lee2, Jungbae Kim2 and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA 99352; 2Korea University, Seoul, Korea

A separation-free quantitative MS-based profiling approach using 2-AA isotopically labeled substrates for high-throughput glycan screening
Justin M. Prien1, Lorna Maheu1, Brad Prater1, and Steve Cockrill1
1Analytical Science, Amgen, Longmont, Colorado 80503

New Strategies for High Pressure-Assisted Digestion in Proteomics
Kim K. Hixson1,2, Daniel López-Ferrer1, Karl K. Weitz1, Ronald J. Moore1, Scott R. Kronewitter1, Heather H. Smallwood3, Mikhail E. Belov1, and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA; 2Washington State University, Pullman, WA; St. Jude Children’s Research Hospital, Memphis, TN

HUPO 2010

Application of Mass Spectrometry-Based Proteomics in Influenza Virus Research and Vaccine Manufacturing
Melkamu Getie-Kebtie; Maryna Eichelberger; Michail Alterman
Center for Biologics Evaluation and Research, FDA, Bethesda, MD, USA

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Eric Bonneil1; Roger Biringer2; Julian Saba2; Andreas Hühmer2; Pierre Thibault1
1IRIC, Universite de Montreal, Montreal, Canada; 2Thermo Fisher Scientific, San Jose, CA

Improved Protocols for Isolation of Intact Mitochondria from Tissue Samples
Vera Gross1; Irina Stavrovskaya2; Sergei Baranov2; Greta Carlson1; Emily Freeman3; Alexander Ivanov3; Bruce Kristal2; Alexander Lazarev1
1Pressure BioSciences, Inc, South Easton, MA; 2Brigham and Women's Hospital; Harvard University, Boston, MA; 3Harvard School of Public Health, Boston, MA

Novel Efficient Alternatives for Sample Preparation Techniques Crucial for Functional Proteomics
Emily Freeman; Alexander Ivanov
Harvard School of Public Health, Boston, MA

Recovery and Immunoaffinity Enrichment of Integral Membrane Proteins from Metastatic Ovarian Cancer Tissue
Luke Schneider; Varsha Likhte; William Wright; Frances Chu; Emma Cambron; Anne Baldwin; Jessica Krakow
Target Discovery, Inc., Palo Alto, CA

BTAW 2010

Multiplex Biomolecule Extraction Using the Pressure Cycling Technology (PCT) Sample Preparation System (PCT SPS)
R. T. Schumacher, M. Potter, A. Lazarev, E. Ting, and N. Lawrence

ABRF 2010

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Roger Biringer1, Eric Bonneil2, Julian Saba1, Andreas Huhmer1, Pierre Thibault2
1Thermo Fisher Scientific, San Jose, CA 2Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Canada
ABRF 2010

A Comparative Study of In-gel Digestions Using Microwave and Pressure Accelerated Technologies
Alvarado, Diana Tran, Bonnie Ching and Brett S. Phinney
University of California Davis

MARM 2010

MALDI and LCMS Protein Biomarkers of Ionizing Radiation
Tiffany Remsen, Vladamir Kachalov, Parth Kothiya, Zeineen A. Momin*, Naresh Vasani, Jason Chouake, Douglas C. Miller, Paul Pevsner
Department of Pathology, University of Missouri School of Medicine Columbia, MO


High Pressure Direct Protein Extraction from Tissue – Trypsin Digest with Pressure Cycling Technology (PCT)
Nicholas Sobol, Jennifer Oprihory*, Parth Kothiya, Zeineen A. Momin, Naresh Vasani, Vladamir Kachalov, Tiffany Remsen, Faraj Al Qaraghuli, Paul H. Pevsner
Department of Pathology University of Missouri School of Medicine, Columbia, MO

Sample Prep 2010 Sample Preparation for Virus,
Toxin & Pathogen Detection & Identification
May 6-7, 2010 Baltimore, MD

Multiplex Biomolecule Extraction Using the Pressure Cycling Technology (PCT) Sample Preparation System (PCT SPS)
R. T. Schumacher, M. Potter, A. Lazarev, E. Ting, and N. Lawrence
Pressure BioSciences, Inc. 14 Norfolk Avenue, S. Easton, MA 02375