Scientific Meetings

XX Plant and Animal Genomics Meeting (PAG)

The PBI Shredder System: A Novel Approach for Affordable and Efficient Sample Preparation Vera Gross¹, Greta Carlson¹, Gary Smejkal², Heather Greenberg³, Bruce Kristal³, Jose’ Lanuza¹, Richard T. Schumacher¹, Nathan Lawrence¹ and Alexander Lazarev^1
1Pressure BioSciences, Inc., South Easton, MA.; ²Harvard Catalyst Laboratory of Innovative Translational Technologies, Boston, MA.;³Brigham and Women's Hospital; Harvard University, Boston, MA.

Integrating SAMPLE PREPARATION Conference 2011

Improved Preparation and Analysis of Biothreat Agents and Infectious Microbial Samples Using Pressure Cycling Technology (PCT)
Bradford Powell, Ph.D., Cernomics Solutions

International Symposium on Human Identification (ISHI) 2011

Application of Pressure Cycling Technology (PCT) in Differential Extraction
Deepthi Nori*, MFS; Dr. Bruce R. McCord, PhD International Forensic Research Institute, Department of Chemistry and Biochemistry Florida International University, Miami, FL

Midwestern Association of Forensics Scientist (MAFS) 2011

Forensic Testing Using Pressure Cycling Technology (PCT)
Thomas E. Strader

The European High Pressure Research Group (49th EHPRG) 2011

HIGH PRESSURE MEDIATED SAMPLE PREPARATION FOR CAPILLARY ELECTROPHORESIS ANALYSIS OF N-LINKED GLYCANS
András Guttman1,2, Zoltán Szabó1 and Barry L. Karger1 1 Barnett Institute, Northeastern University, Boston, MA 02115 2 HLBS, University of Debrecen, Hungary 49th EHPRG, Aug 28-Sept 2, 2011 Budapest, Hungary

Environmentally Benign Reduction of Organic Compounds in Water under High Hydrostatic Pressure
Anna Tomin, Alexander Lazarev, Bela Torok Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

TRENDS AND APPLICATIONS OF HYDROSTATIC PRESSURE IN BIOMEDICAL ANALYSIS: EXPLORING A FORGOTTEN THERMODYNAMIC DIMENSION
Alexander Lazarev
Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

Development and Applications of the Automated Benchtop Dynamic Pressure Generator for High Pressure Bioscience Research
Alexander Lazarev, Timothy Straub, James Behnke and Edmund Y. Ting
Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

Protein Society Meeting 2011

Development and applications of the automated benchtop dynamic pressure generator for high pressure perturbation of protein structure and protein dynamics studies
A. Lazarev, T. Straub, J. Behnke, E. Y. Ting
Pressure BioSciences Inc., Boston, MA, USA

American Society for Microbiology (ASM) 2011

Composition of Microbial Communities in Petroleum Deposits
Jamie Scott, Debarati Paul, Magan Green, Lewis R. Brown, and Mark L. Lawrence.
College of Veterinary Medicine, Biological Sciences, Mississippi State University, Mississippi State, MS-39762
ASM 2011

American Society for Mass Spectrometry (ASMS) 2011

Global Deglycosylation in High Throughput LC-MS Glycomics: Comparing Microwave, Pressure, and Ultrasound Effects on PNGase F with Human Serum
Scott R. Kronewitter, Gordon W. Slysz, Daniel López-Ferrer, Matthew E. Monroe, Rui Zhao, Thomas L. Fillmore, Karl K. Weitz, Robert A. Heegel, Daniel J. Orton, Brian L. LaMarche, Kevin L. Crowell, Anuj R. Shah, Carrie D. Nicora, Heather M. Brewer, David G. Camp II, Gordon A. Anderson, Ronald J. Moore, and Richard D. Smith

Innovative Sample Preparation Tools for Proteomics and Analytical Laboratories
Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375

The Evaluation of Pressure-Assisted Enzymatic Digestion for the Optimal Digestion of Monoclonal Antibodies
Yazen Jmeian; Eric Beil; Dariusz Janecki; Steven C. Pomerantz; Jennifer F. Nemeth
Centocor R&D, a division of J&JPRD LLC, Radnor, PA

Does LC Separation of Intact Proteins Hinder Identification of Bacterial Markers?
Melinda A. McFarland ; Denis Andrzejewski; John H. Callahan; Steven M. Musser
FDA-CFSAN, College Park, MD

Development of a 20 kpsi Enzymatic Digester for High Throughput Proteomic Analysis and Its Application to Membrane Proteomics
Seok-Won Hyung¹; Daniel López-Ferrer¹; Daniel J. Orton¹; Erika Zink¹; Karl K. Weitz¹; Rui Zhao¹; Ronald J. Moore¹; Kim K. Hixson¹; Edmund Y. Ting²; Alexander V. Lazarev²; Richard D. Smith^1
1Pacific Northwest National Laboratory, Richland, WA; 2Pressure Biosciences, Inc, South Easton, MA

Identification and Validation of Vaccine Candidate Proteins by 2-Dimensional Mass Spectrometry Analysis of Group A Streptococcus
Lori C Stansberry; Angela Payne; Julie Skinner; Loren Schultz; Jon Heinrichs; Eberhard Durr
Merck and Co., West Point, PA

US HUPO 2011

Low Energy Mechanical Shredding: A New Approach for a Wide Range of Sample Preparation Needs
Vera Gross1, Greta Carlson1, Gary Smejkal2. Heather Greenberg3, Bruce Kristal3, Luke Schneider4 and Alexander Lazarev1
1Pressure BioSciences, Inc., South Easton, MA.; 2Harvard Catalyst Laboratory of Innovative Translational Technologies, Boston, MA.;
3Brigham and Women's Hospital; Harvard University, Boston, MA.; 4Target Discovery, Inc., Palo Alto, CA.

ABRF 2011

Effective Semi-Automated Extraction of Intact Mitochondria from Solid Tissues Using Gentle Mechanical Homogenization and Pressure Cycling Technology
Vera S. Gross1, Greta Carlson1, Emily Freeman2, Alexander R. Ivanov2, Heather Greenberg3, Sergei Baranov3, Irina Stavrovskaya3, Bruce Kristal3, Alexander Lazarev1
1Pressure BioSciences, Inc., South Easton, MA. 2HSPH Proteomics Resource, Harvard School of Public Health, Boston, MA. 3Brigham and Women's Hospital; Harvard University, Boston, MA

AAFS 2011

Posters

Poster: Application of Pressure Cycling Technology (PCT) in Differential Extraction
Deepthi Nori*, MFS; Dr. Bruce R. McCord, PhD
International Forensic Research Institute, Department of Chemistry and Biochemistry
Florida International University, Miami, FL USA

Seminar : Application of Pressure Cycling Technology (PCT) in Differential Extraction
Deepthi Nori*, MFS; Dr. Bruce R. McCord, PhD
International Forensic Research Institute, Department of Chemistry and Biochemistry
Florida International University, Miami, FL USA

Mass Spectrometry Application for the Clinical Laboratory (MSACL 2011)

Video

PCT Product Pipeline

Posters

MALDI and LCMS Identifications of Early Protein and LA-ICP-MS Identification of Early Metal Biomarkers of Ionizing Radiation
P. Pevsner1, G. Li2, N. Sobol1, D.C Miller1, D. Robertson2,3
1Department of Pathology and Anatomical Sciences
University of Missouri School of Medicine
2 University of Missouri Department of Chemistry
3University of Missouri Research Reactor (MURR)
Columbia, MO, USA

Rapid Protein Extraction and Trypsin Digestion with Pressure Cycling Technology (PCT)
Paul H Pevsner et al., University of Missouri School of Medicine

Benign vs. Malignant Colorectal Polyps-Mass Spectrometry Diagnosis
Paul H Pevsner et al., University of Missouri School of Medicine

Improving the Efficiency and Throughput of an Enzymatic Digestion of Klotho using Pressure Cycling Technology (PCT)
Taha Rezai1, Vera Gross2, Alexander Lazarev2, Mary Lopez1
1Thermo Fisher Scientific, Cambridge, MA; 2Pressure BioSciences Inc., Boston, MA
MSACL 2011

Intact and Functional Mitochondria from Solid Tissue: Effective Semi-Automated Extraction Using Gentle Mechanical Homogenization and Pressure Cycling Technology
Alexander Lazarev1, Vera S. Gross1, Greta Carlson1, Emily Freeman2, Alexander R. Ivanov2, Heather Greenberg3, Sergei Baranov3, Irina Stavrovskaya3, Bruce Kristal3 1Pressure BioSciences, Inc., South Easton, MA. 2HSPH Proteomics Resource, Harvard School of Public Health, Boston, MA. 3Brigham and Women's Hospital; Harvard University, Boston, MA

Lunch and Learn Seminars

Applications of Pressure Cycling Technology (PCT) in Biotechnology
Dr. Vera Gross, Senior Scientist, Pressure BioSciences, Inc.

Digestion with Pressure Cycling Technology (PCT)
Nicholas Sobol, Jennifer Oprihory*, Tiffany Remsen, A. Yuen, P., Paul Pevsner.
Department of Pathology and Anatomical Sciences
University of Missouri School of Medicine, Columbia, MO

Applications of High Pressure for Automated High Performance Proteomics Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes
Daniel López-Ferrer
Caprion Proteomics US LLC

Recovery and Immunoaffinity Mass Spectrometric Analysis of Integral Membrane Proteins from Metastatic Ovarian Cancer Tissue Using PCT™ and ProteoSolve™-TD Buffers
Dr. Luke V. Schneider, CSO, Target Discovery, Inc.

Barocycler HUB440, Barocycler FFPE & Protein Extraction Service, XStreamPCT™ HPLC Platform Digestion Module, and the Barocycler HT48-384: a Peek into the PBI Product Pipeline and Opportunities for Partnering
Dr. Edmund Y. Ting, Sr. Vice President of Engineering, Pressure BioSciences, Inc
Mr. Richard T. Schumacher, President and CEO, Pressure BioSciences, Inc.

Association for Laboratory Automation (ALA 2011)

Seminar: The Challenges of Automating Sample Preparation in the Proteomics Era
Nathan P. Lawrence, Ph.D. V.P. Marketing, PBI

Harvard Symposium

Symposium: Agenda and Abstracts: Applications of Ultra-High Pressure in Biotechnology
Harvard Medical School Joseph B. Martin Conference Center

Slide Presentations

(Fundamentals of) High-pressure Instruments for Innovation and Discovery
Edmund Ting, Sc.D., Pressure BioSciences

Development of Essential Sample Preparation Techniques in Proteomics Using Ultra-High Pressure
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, HSPH Proteomics Resource, Department of Genetics and Complex Diseases, Boston, MA 2115

Comparison between ultra-high pressure and atmospheric tryptic digestion for proteomic analyses
E. Bonneil1, R. Biringer2, C. Bell1, P. Thibault1
1Institute for Research in Immunology and Cancer Université de Montréal; 2Thermo Fisher Scientific

Development of new technology approaches for high-throughput proteome analysis
Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes
Daniel López-Ferrer, Biological Separations & Mass Spectrometry Group, Pacific Northwest National Laboratory

High Pressure Direct Protein Extraction from Tissue – Trypsin Digest with Pressure Cycling Technology (PCT)
Paul H. , Jennifer Oprihory, Nicholas Sobol, Parth Kothiya, Zeineen A. Momin, Naresh Vasani, and Tiffany Remsen
Department of Pathology, University of Missouri School of Medicine, Columbia, MO

Membrane Protein Extraction and Biomarker Analysis from Solid Metastatic Ovarian Tumors with ProteoSolve™-TD Buffers and Pressure Cycling Technology
Dr. Luke V. Schneider, CSO, Target Discovery, Inc.

A Need for Improved Sample Inactivation and Extraction Methods to Support Systems Biology Analysis of Biological Threat Organisms
1 Bradford S. Powell and 2 Loreen L. Lofts
1 Bacteriology Division, 2 Virology Division
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702

Convenient and Reliable Extraction of Borrelia burgdorferi from Ticks Using Pressure Cycling Technology and the PCT Shredder
Richard C. Tilton, Ph.D.
Medical Diagnostics Laboratories

Pressure Cycling Technology (PCT) Augments Sensitivity of Detection and Robustness in Forensic DNA Analyses
Bruce Budowle
Department of Forensic and Investigative Genetics, Institute of Investigative Genetics, University of North Texas Health Science Center, Ft. Worth, Texas

RAPID SAMPLE PREPARATION METHODS FOR THE ANALYSIS OF N-LINKED GLYCANS
Zoltan Szabo, András Guttman, Tomas Rejtar and Barry L. Karger
Barnett Institute, Boston, MA, USA

High-Pressure Assisted In-Gel Tryptic Digestion for Qualitative and Quantitative Characterization of Protein Mixtures
Melkamu Getie-Kebtie and Michael Alterman
FDA-CBER-NIH, Rockville Pike, HFM – 735 Bldg 29A rm 2B20, Bethesda, MD 20892

High Pressure in Life Sciences – Trends and Future Opportunities
Alexander Lazarev, PhD, Pressure BioSciences, Inc.

ASCB 2010

Simple Protocols for Isolation of Intact Mitochondria Using The SHREDDER SG3 and PBI Mitochondria Isolation Kits
Pressure BioSciences, Inc.

21st International Symposium on Human Identification

PRESSURE CYCLING TECHNOLOGY (PCT) APPLICATIONS FOR FORENSIC DNA ANALYSIS
Pam Marshall, MS; Jonathan King, MS; Meredith Turnbough, PhD; Arthur J. Eisenberg, PhD; Bruce Budowle, PhD
Institute of Investigative Genetics, Department of Forensics and Investigative Genetics,
University of North Texas Health Science Center, Fort Worth, Texas

Application of Pressure Cycling Technology (PCT) in Differential Extraction
Deepthi Nori, MFS** Bruce R. McCord, PhD
International Forensic Research Institute, Department of Chemistry and Biochemistry
Florida International University, Miami, FL 33199

BioPharm America 2010

Pressure Cycling Technology (PCT): A Novel, Enabling Platform Revolutionizing Biomarker Discovery
Richard T. Schumacher, PBI CEO and President

The Second Annual Current and Future Advances in Human Identification Conference

Pressure Cycling Technology (PCT): Potential Applications in Forensic Science
Nathan Lawrence
Pressure BioSciences, Inc., 14 Norfolk Ave., S. Easton, MA 02375

High Pressure Bioscience and Biotechnology (HPBB2010)

Optimization of high pressure-assisted methods for proteomic sample preparation
Alexander V. Lazarev1*; Vera S. Gross1; Greta Carlson1; Edmund Ting1; Emily Freeman2;
Alexander R. Ivanov2 ; Melkamu Getie-Kebtie3; Michail Alterman3
1Pressure BioSciences, South Easton, MA, USA; 2Harvard School of Public Health, Boston, MA, USA; 3FDA, CBER
Rockville , MD, USA

APS 2010

Improved Extraction of DNA of Ca. Liberibacter Species from Plants and Cultivated Cells Using Pressure Cycling Technology
A. Sechler1, A Marques2, N. Lawrence3, N. W. Schaad1
1FDWSRU, USDA-ARS, Fort Detrick, MD USA
2EMBRAPA, Brasilia, Brasil
3Pressure BioSciences Inc., South Easton, MA, USA

Neuronal Development, Synaptic Function & Behavior C. elegans 2010

Pressure mediated scheme for isolating native proteins from Caenorhabditis elegans under mild non-denaturing conditions
Gary B. Smejkal1,2, Thomas E. Strader3, W. Kelley Thomas1, Jobriah Anderson1, Krystalynne Morris1, and Winston P. Kuo2
1 Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH. 2 Harvard Medical School, Harvard Catalyst, Laboratory for Innovative Translational Technologies, Boston, MA. 3 Pressure BioSciences, South Easton, MA

ASMS 2010

Novel efficient alternatives for essential sample preparation techniques in functional proteomics
Emily Freeman and Alexander R. Ivanov
Harvard School of Public Health, Department of Genetics and Complex Diseases

Tandem Mass Spectrometry Analysis of Ex Vivo Amyloid Fibril and Tissue Samples
Zhenning Hong,1Giuseppe Infusini,1Lawreen H. Connors,2Martha Skinner,2Catherine E. Costello1,2
Mass Spectrometry Resource and 2Amyloid Treatment and Research Program, Boston University School of Medicine, Boston, MA USA

Proteomic Comparison of Sun-protected vs -Exposed and Young vs Gently-Aged Human Epithelium
1 Facility Center California R.A. Eigenheer1, J.T. Smilowitz2 and B.S. Phinney1
1. Proteomics Core Facility, Genome Center, University of California, Davis
2. Department of Food Science and Technology, University of California, Davis

High Throughput Pressure-Enhanced Protein Extraction and Enzymatic Digestion With Pressure Cycling Technology (PCT) and PCT MicroTubes
C. Dussault, G. Carlson, V. Gross, N. Lawrence, A. Lazarev, M. Potter, R. T. Schumacher, and E. Ting
Pressure BioSciences, Inc. 14 Norfolk Avenue, S. Easton, MA 02375

Screening for Drug-Drug Interactions Using a Targeted Proteomics Strategy
Daniel B. Kassel1; Kheng B. Lim1; Melinda Manuel1;
Teruaki Okuda2; Naomi Kamiguchi2; Christie L Hunter3; Brian Williamson3; Lydia Nuwaysir3
1Takeda San Diego, Inc, San Diego, CA; 2Takeda Pharmaceutical Company, Limited, Osaka, Japan;
3AB Sciex, Foster City, CA

Application of High Pressure and Highly Stable Trypsin Aggregate Coating on Superparamagnetic Magnetite/Silica Nanoparticles for High Performance Proteomics
Daniel López-Ferrer1, Byoungsoo Lee2, Jungbae Kim2 and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA 99352; 2Korea University, Seoul, Korea

A separation-free quantitative MS-based profiling approach using 2-AA isotopically labeled substrates for high-throughput glycan screening
Justin M. Prien1, Lorna Maheu1, Brad Prater1, and Steve Cockrill1
1Analytical Science, Amgen, Longmont, Colorado 80503

New Strategies for High Pressure-Assisted Digestion in Proteomics
Kim K. Hixson1,2, Daniel López-Ferrer1, Karl K. Weitz1, Ronald J. Moore1, Scott R. Kronewitter1, Heather H. Smallwood3, Mikhail E. Belov1, and Richard D. Smith1
1Pacific Northwest National Laboratory, Richland, WA; 2Washington State University, Pullman, WA; St. Jude Children’s Research Hospital, Memphis, TN

HUPO 2010

Application of Mass Spectrometry-Based Proteomics in Influenza Virus Research and Vaccine Manufacturing
Melkamu Getie-Kebtie; Maryna Eichelberger; Michail Alterman
Center for Biologics Evaluation and Research, FDA, Bethesda, MD, USA

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Eric Bonneil1; Roger Biringer2; Julian Saba2; Andreas Hühmer2; Pierre Thibault1
1IRIC, Universite de Montreal, Montreal, Canada; 2Thermo Fisher Scientific, San Jose, CA

Improved Protocols for Isolation of Intact Mitochondria from Tissue Samples
Vera Gross1; Irina Stavrovskaya2; Sergei Baranov2; Greta Carlson1; Emily Freeman3; Alexander Ivanov3; Bruce Kristal2; Alexander Lazarev1
1Pressure BioSciences, Inc, South Easton, MA; 2Brigham and Women's Hospital; Harvard University, Boston, MA; 3Harvard School of Public Health, Boston, MA

Novel Efficient Alternatives for Sample Preparation Techniques Crucial for Functional Proteomics
Emily Freeman; Alexander Ivanov
Harvard School of Public Health, Boston, MA

Recovery and Immunoaffinity Enrichment of Integral Membrane Proteins from Metastatic Ovarian Cancer Tissue
Luke Schneider; Varsha Likhte; William Wright; Frances Chu; Emma Cambron; Anne Baldwin; Jessica Krakow
Target Discovery, Inc., Palo Alto, CA

BTAW 2010

Multiplex Biomolecule Extraction Using the Pressure Cycling Technology (PCT) Sample Preparation System (PCT SPS)
R. T. Schumacher, M. Potter, A. Lazarev, E. Ting, and N. Lawrence

ABRF 2010

The Effect of Pressure Cycling on Proteolytic Cleavage Efficiency, Reaction Time and Protein Sequence Coverage
Roger Biringer1, Eric Bonneil2, Julian Saba1, Andreas Huhmer1, Pierre Thibault2
1Thermo Fisher Scientific, San Jose, CA 2Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Canada
ABRF 2010

A Comparative Study of In-gel Digestions Using Microwave and Pressure Accelerated Technologies
Alvarado, Diana Tran, Bonnie Ching and Brett S. Phinney
University of California Davis

MARM 2010

MALDI and LCMS Protein Biomarkers of Ionizing Radiation
Tiffany Remsen, Vladamir Kachalov, Parth Kothiya, Zeineen A. Momin*, Naresh Vasani, Jason Chouake, Douglas C. Miller, Paul Pevsner
Department of Pathology, University of Missouri School of Medicine Columbia, MO


High Pressure Direct Protein Extraction from Tissue – Trypsin Digest with Pressure Cycling Technology (PCT)
Nicholas Sobol, Jennifer Oprihory*, Parth Kothiya, Zeineen A. Momin, Naresh Vasani, Vladamir Kachalov, Tiffany Remsen, Faraj Al Qaraghuli, Paul H. Pevsner
Department of Pathology University of Missouri School of Medicine, Columbia, MO

Sample Prep 2010 Sample Preparation for Virus,
Toxin & Pathogen Detection & Identification
May 6-7, 2010 Baltimore, MD

Multiplex Biomolecule Extraction Using the Pressure Cycling Technology (PCT) Sample Preparation System (PCT SPS)
R. T. Schumacher, M. Potter, A. Lazarev, E. Ting, and N. Lawrence
Pressure BioSciences, Inc. 14 Norfolk Avenue, S. Easton, MA 02375