2004-2009 PBI Third Party Publications Archive
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Simple Protocols for Isolation of Intact Mitochondria Using The SHREDDER SG3 and PBI Mitochondria Isolation Kits
Pressure BioSciences, South Easton, MA 02375
- Optimization of high pressure-assisted methods for proteomic sample preparation
Alexander V. Lazarev1*; Vera S. Gross1; Greta Carlson1; Edmund Ting1; Emily Freeman2;
Alexander R. Ivanov2 ; Melkamu Getie-Kebtie3; Michail Alterman3
1Pressure BioSciences, South Easton, MA, USA; 2Harvard School of Public Health, Boston, MA, USA; 3FDA, CBER
Rockville , MD, USA
- DNA EXTRACTION FROM HAIR USING
PRESSURE CYCLING TECHNOLOGY
Elizabeth Feller, MS; Suzanne Gonzalez, PhD; Amy Smuts, MS; Bruce Budowle, PhD; Arthur J. Eisenberg, PhD
- Phosphopeptide isolation from Caenorhabditis elegans using the ProteoSOLVE, PCT, and PhosphoScan Technologies
Gabrielle E. Giese, Gary B. Smejkal, Feixia Chu, John J. Collins, and Winston P. Kuo
- Thermal inactivation prior to 2DGE stabilizes brain proteome
Gary B. Smejkal, Chiara Rivas-Morello, Jae-Hyung Robert Chang, Alexander J. Trachtenberg, John Lindsay, Mats Borén, Alexander Lazarev and Winston P. Kuo
- Detection of differential protein expression between individual Daphnia by Pressure Cycling Technology (PCT) and two-dimensional gel electrophoresis
Darren J. Bauer, Gary B. Smejkal, W. Kelley Thomas, Myra Robinson and Winston P. Kuo
-
Incidence and spatial distribution of Rhizoctonia and Pythium species determined with real-time PCR
K. L. SCHROEDER, T. C. Paulitz, and P. A. Okubara USDA-ARS, Washington State University, Pullman, WA 99164-6430
-
Top-Down Identification of Bacterial Intact Protein Expression Profile Markers
Melinda A. McFarland; John H. Callahan; Denis Andrzejewski; Rebecca Bell; Steven M. Musser
FDA/CFSAN, College Park, MD - ASMS 2009
-
Discovery of Mitochondrial Protein Biomarkers of Atrial Fibrillation Using Unique Human Tissue Samples
Maryam Goudarzi1, Mark M. Ross1, Weidong Zhou1, Amy Van Meter1, Emanuel Petricoin1, Lance Liotta1, Lisa Martin2, Chidima Martin2 and Niv Ad2
1George Mason University, Manassas, VA; 2Inova Heart & Vascular Institute, Falls Church, VA
-
Pressure Enhanced Processing (PrEP) using The PCT Shredder and Pressure Cycling Technology (PCT)
maximizes protein yields from the nematode Caenorhabditis elegans under mild, nondenaturing conditions
W. Kelley Thomas, Gary B. Smejkal, Jobriah Anderson, Myra H. Robinson, KrystalynneMorris, and Richard T. Schumacher
- Searching for efficient and high-throughput alternatives for essential sample preparation
techniques in mass spectrometry-based functional proteomics
Emily Freeman; Yelena Margolin; Alexander R. Ivanov
- Pressure Cycling for Sample Preparation
PCT System Provides Automated Alternative to Manual Methods
Richard T. Schumacher, Vera Gross, Ph.D., Edmund Y. Ting, ScD., and Alexander Lazarev, Ph.D
- Complete Disruption of Caenorhabditis elegans Under Non-denaturing Conditions Using Pressure Cycling Technology (PCT)
- Soluble Forms of the Notch Ligands Delta1 and
Jagged1 Promote in Vivo Tumorigenicity in NIH3T3
Fibroblasts with Distinct Phenotypes
Sumithra Urs, Alice Roudabush,
Christine F. O’Neill, Ilka Pinz, Igor Prudovsky,
Doreen Kacer, Yuefang Tang, Lucy Liaw,
and Deena Small
- Pilot Proteomic Profile of Differentially Regulated Proteins in Right Atrial Appendage Before and After Cardiac Surgery Using Cardioplegia and Cardiopulmonary Bypass
Richard T. Clements, Gary Smejkal, Neel R. Sodha, Alexander R. Ivanov, John M. Asara, Jun Feng, Alexander Lazarev, Shiva Gautam, Venkatachalam Senthilnathan, Kamal R. Khabbaz, Cesario Bianchi and Frank W. Sellke
- Application of pressurized solvents for ultra fast proteolysis: Proteomics on the fly
DANIEL LOPEZ FERRER; Konstantinos Petritis; Natacha M Lourette; Brian H. Clowers; Kim K. Hixson; Tyler H
Heibeck; Eric A. Livesay; Ryan Kelly; David Prior; Ljiljana Pasa-tolic; David G. Camp; Mikhail Belov; Richard D. Smith
Pacific Northwest National Laboratory, Richland, WA
- Intact Protein Liquid Chromatography Mass Spectrometry for Bacteria Strain Differentiation and
Bacterial Toxin Detection
John H. Callahan; Denis Andrzejewski; Rebecca Bell; Eric Brown; Steve Musser FDA/CFSAN, College Park, MD
- MALDI Mass Spectrometry Identification of Proteins in a Murine Transgenic Model of Apert Syndrome
G. Rothschild, A. Mansukhani, C. Basilico and P. Pevsner
- Growth Inhibition of Retinoic Acid Treated MCF-7 Breast Cancer Cells-Identification of Sox 9 and Other Proteins
T Remsen, P Kessler, A Stern, H Samuels and P Pevsner
- Fourier Transform Mass Spectrometry Identification of Disease Biomarker Proteins
P. Pevsner, G. Kruppa, T. Remsen, F. Naftolin, P. Kessler and A. Stern
- LCMS Identification of In Vitro Fertilization Culture Media Proteins
P. Pevsner, S. Talebian, T. Remsen, F. Naftolin, P Kessler, A Stern, F. Licciardi and J. Grifo
- Mass Spectrometry of Buccal Mucosa -Biomarkers for Biodosimetry
in Radiation Incidents
P.H. Pevsner, S. Formenti, T. Remsen, G. Kruppa,P. Kessler, G. Rothschild
Jorge Ghiso, J. Melamed, B. S. Rosenstein, R. Schneider, F. Naftolin and A. Stern
- Partial-Body Radiation Diagnostic Biomarkers and Medical Management of Radiation Injury Workshop
P.H. Pevsner, S. Formenti, T. Remsen, G. Kruppa, P. Kessler, G. Rothschild, Jorge Ghiso, J. Melamed,
B. S. Rosenstein, R. Schneider, F. Naftolin, and A. Stern
- Strategies to recover proteins from ocular tissues for proteomics
Nikhil Patel, Ekta Solanki, Renata Picciani, Valerie Cavett , Jennifer A. Caldwell-Busby, Sanjoy K. Bhattacharya, Dr.
- Tissue Fractionation by Hydrostatic Pressure Cycling Technology:
The Unified Sample Preparation Technique for Systems Biology Studies
Vera Gross, Greta Carlson, Ada T Kwan, Gary Smejkal, Emily Freeman, Alexander R Ivanov, Alexander Lazarev
- Isolation of Mitochondria from Cell Cultures by
PCT for Proteomic Analysis
Vera Gross, Alexander V. Lazarev, Nathan P. Lawrence, and Richard T. Schumacher
Pressure BioSciences, Inc., South Easton, MA
- Smejkal & Schweitzer ERP article published: "Will current technologies enable dinosaur proteomics?
Gary B. Smejkal, Mary H. Schweitzer
Future Drugs, Ltd. 2007
- Improved extraction of Rhizoctonia and Pythium DNA from wheat roots and soil samples using pressure cycling technology
Patricia A. Okubara, Chunqin Li, Kurtis L. Schroeder, Richard T. Schumacher, and Nathan P. Lawerence
- Growth Inhibition of Retinoic Acid Treated MCF-7 Breast Cancer Cells-Identiication of Sox 9
and other Proteins
P. Pevsner, D. Vecchione, T. Remsen, P. Kessler, N. Levers, P. Yang, A. Stern, H. Samuels
Department of Pharmacology, New York University School of Medicine, New York, NY, US
- Colorectal Carcinoma - Field Defects in Satellite Tissue
P. Pevsner, D. Vecchione, T. Remsen, P. Kessler, M. Momeni, S. Duddempudi, F. Francois, A. Stern, S. Anand
Department of Pharmacology, Department of Medicine, New York University School of Medicine, New York, NY, US
The Brooklyn Hospital Center, Brooklyn, New York, US
- in-Vitro Fertilization Growth Media Proteins in Competent Embryos
P. Pevsner, F. Naftolin, D. Vecchione, T. Remsen, S. Talebian, P. Kessler, F. Licciardi, and J. Grifo
Department of Pharmacology, Department of Obstetrics and Gynecology, Reproductive Endocrinology and Infertility
New York University School of Medicine, New York, NY, US
- Stroke - Prophylactic Estrogen (Estradiol) Therapy GI 200038, neurofilament-L
David Vecchione, Frederick Naftolin,Tiffany Remsen, Paul Kessler, Arnold Stern, Paul H. Pevsner
Dept of Pharmacology, Department of Obstetrics and Gynecology, New York University School of Medicine, New York, NY, US
- Application of pressure-cycling technology to RNA extraction from Legionella pneumophila cells
Mourad FERHAT, Claire ANDREA, Christophe GILBERT and Daniele ATLAN
- A Systems Biology Approach to the Pathogenesis of Obesity-related Nonalcoholic Fatty Liver Disease Using Reverse Phase Protein Microarrays for Multiplexed Cell Signaling Analysis
Valerie S. Calvert, Rochelle Collantes, Hazem Elariny, Arian Afendy, Ancha Baranova, Michael Mendoza, Zachary Goodman, Lance A. Liotta, Emanuel F. Petricoin, and Zobair M. Younossi
- Tris interference in IEF and 2-DE
Gary B. Smejkal, Myra H. Robinson
ELECTROPHORESIS May 10 2007, Volume 28, Issue 10, Pages: 1601-1606
- Proteomic profile of differentially regulated proteins in human myocardium before
and after cardiac surgery utilizing cardioplegia and cardiopulmonary bypass.
Richard T. Clements, Neel R. Sodha, Gary Smejkal, Jun Feng, Sirisha Emani, Venkatachalam Senthilnathan,
Alexander Lazarev, Kamal R. Khabbaz, Cesario Bianchi, and Frank W. Sellke.
- Colon Cancer: Protein Biomarkers in Tissue and Body Fluids
M. Momeni, MD, P. Pevsner, MD, D. Vecchione, BS, B. Stall,AAS, K. Zaalook, MD, S. Duddempudi, MD, M. Joseph,
Department of Gastroeteroogy
- Microtubule Associated Proteins (MAP) and Motor Molecules: Direct Tissue
MALDI Identification and Imaging
P. Pevsner, F. Naftolin, D. Vecchione, B. Stall, D. Miller, P. Kessler, A. Stern
- The Daphnia Genomics Consortium Meeting: The Genome Biology of the Model
Crustacean Daphnia
Darren J Bauer
- "How it works. Extraction of DNA from Bone Samples."
Froelich, P.
Forensics Magazine, February/March 2007, Vol. 4 No. 1 p.55
- Pressure Cycling Technology (PCT) Facilitates Analysis of the Frankia Proteome
Louis S. Tisa, Gary B. Smejkal, Ada Kwan, Ilyana Romanovsky and Alexander Lazarev
University of New Hampshire, Department of Microbiology, Durham, NH and Pressure BioSciences, Proteomics and Small Molecules Applications Laboratory, Woburn, MA.
- Living Large: Elucidation of the Frankia EAN1pec Genome Sequence Shows Gene
Expansion and Metabolic Versatility
Louis S Tisa, David R Benson, Gary B. Smejkal, Pascal Lapierre, J. Peter Gogarten, Philippe Normand, M. Pilar Francino, and Paul Richardson
Dept. Microbiology , U New Hampshire, Durham, NH, USA; Dept. Mol. Cell Biol., U Connecticut , Storrs, CT, USA; JGI, Walnut Creek, CA, USA, Pressure Biosciences, Inc, Bridgewater, MA, USA, Ecologie Microbienne UMR CNRS 5557, Université Lyon, Villeurbanne, France
- Not the Same Old Grind
Chongwoo Yu and Lucinda H. Cohen
Pfizer Global Research and Development, Ann Arbor, Michigan, USA.
- Pressure Cycling Technology Sample Preparation System (PCT SPS) Improves
Quantification of Pathogen DNA in Plants and Soil
Patricia Okubara, Chunqin Li, Kurtis Schroeder, and Nathan Lawrence
USDA ARS, Root Disease & Biological Control Research Unit, Pullman, WA Pressure BioSciences, Inc., West Bridgewater, MA
- Solution phase isoelectric fractionation in the multicompartment electrolyser: A divide and conquer strategy for the analysis of complex proteomes
Gary B. Smejkal, Alexander Lazarev
BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. VOL 4. NO 1. 76–81. MAY 2005
- Pressure Cycling Technology (PCT) Applications in Extraction of Biomolecules from Challenging Biological Sample
Feng Tao, Chunqin Li, Gary Smejkal, Alexander Lazarev, Nathan Lawrence, and Richard T. Schumacher
Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology , Vol. 1, 166-173, 2007
- Proteomic Analyses of Proteins Isolated From Mammalian Bone
Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier
HUPO 6th Annual World Congress Seoul, October 6 – 10, 2007
- "Pressure Cycling Technology (PCT): a Novel Sample Preparation Approach to Biomarker Discovery and Drug Development in Lipid-Rich Samples"
Ilyana Romanovsky, Gary Smejkal, Alexander Lazarev, Nate Lawrence, and Richard T. Schumacher
Next Generation Pharmaceutical
- Application of pressure cycling technology to tissue sample preparation for 2-DE
Heather Ringham, Richard L. Bell, Gary B. Smejkal, James Behnke, Frank A. Witzmann
ELECTROPHORESIS, March 6 2007, Volume 28, Issue 6, Pages: 1022-1024
Pressure Cycling for Sample Preparation
PCT System Provides Automated Alternative to Manual Methods
Richard T. Schumacher, Vera Gross, Ph.D., Edmund Y. Ting, ScD., and Alexander Lazarev, Ph.D
Complete Disruption of Caenorhabditis elegans Under Non-denaturing Conditions Using Pressure Cycling Technology (PCT)
Tissue Fractionation by Hydrostatic Pressure Cycling Technology:
The Unified Sample Preparation Technique for Systems Biology Studies
Vera Gross, Greta Carlson, Ada T Kwan, Gary Smejkal, Emily Freeman, Alexander R Ivanov, Alexander Lazarev
Isolation of Mitochondria from Cell Cultures by
PCT for Proteomic Analysis
Vera Gross, Alexander V. Lazarev, Nathan P. Lawrence, and Richard T. Schumacher
Pressure Cycling Technology (PCT) mediated sample preparation schemes for isolating proteins from the nematode Caenorhabditis elegans
G.B. Smejkal, J. Anderson, W.K. Thomas, M.H. Robinson, A.T. Kwan, G. Carlson, V. Gross, R.T. Schumacher and A.V. Lazarev (2008)
"Isolation of Proteins from Small Subcutaneous Murine Tumor and Adipose Tissues Using a Pressure Cycling Technology"
Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, Sumithra Urs, and Deena Small (2008)
"Tissue Fractionation by Hydrostatic Pressure Cycling:
The Unified Sample Preparation Technique for Systems Biology Studies"
Vera Gross, Greta Carlson, Gary Smejkal, Ada Kwan, Emily Freeman,Alexander R. Ivanov and Alexander Lazarev (2008)
"Pressure Cycling Technology (PCT) mediated sample preparation Pressure Cycling Technology (PCT) mediated sample preparation schemes enabling high quality two-dimensional gel electrophoresis of Fagus grandifolia tree bark proteins"
Gary Smejkal, AdaKwan, Michael Zianni, David Mandich, Mary Mason, and Jennifer Koch (2008)
Gary Smejkal, Ada Kwan, Vera Gross, Alexander Lazarev, Kelley Thomas, Darren Bauer,
Richard Clements, Cesario Bianchi, Frank Sellke, Michael Zianni, David Mandich, Mary Morgan, and Jennifer Koch (2007)
"The Evolution of Sample Preparation in Proteomics:
Applications of Pressure Cycling Technology (PCT) Spanning from Bacteria to Homo sapiens"
Gary B. Smejkal, W. Kelley Thomas, Darren Bauer, Michael Kinter, Ada Kwan, Frank Witzmann, and Heather Ringham
(2007)
"Proteomic analysis of individual Daphnia microcrustaceans"
Alexander Lazarev, Gary Smejkal, Ilyana Romanovsky, Haiming Cao, Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Adipose Tissue Using Detergent-Free Protein Extraction by Pressure Cycling and High Resolution Tandem Mass Spectrometry"
Gary B. Smejkal, Ada T. Kwan, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier (2007)
"Electrophoretic Analyses of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling Technology"
-9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry, Cambridge, MA
Alexander Lazarev; Gary Smejkal; Ilyana Romanovsky; Ada Kwan; Haiming Cao; Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Murine Adipose Tissue Using Pressure Cycling Technology and High Resolution Tandem Mass Spectrometry U.S. HUPO (2007)"
Gary B. Smejkal, D. C. Muddiman, M. H. Schweitzer, T. S. Collier and A. T. Kwan (2007)
"Electrophoretic Analyses of Proteins and Peptides Isolated From Cortical Bone Using a Pressure Cycling Technology"
-Third Annual US HUPO Conference, Seattle WA.
- Gary Smejkal, Ada Kwan, Vera Gross, Alexander Lazarev, Kelley Thomas, Darren Bauer,
Richard Clements, Cesario Bianchi, Frank Sellke, Michael Zianni, David Mandich, Mary Morgan, and Jennifer Koch (2007)
"The Evolution of Sample Preparation in Proteomics:
Applications of Pressure Cycling Technology (PCT) Spanning from Bacteria to Homo sapiens"
- Alexander Lazarev, Gary Smejkal, Ilyana Romanovsky, Haiming Cao, Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Adipose Tissue Using Detergent-Free Protein Extraction by Pressure Cycling and High Resolution Tandem Mass Spectrometry"
- Gary B. Smejkal, Ada T. Kwan, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier (2007)
"Electrophoretic Analyses of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling Technology"
-9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry, Cambridge, MA
- Alexander Lazarev; Gary Smejkal; Ilyana Romanovsky; Ada Kwan; Haiming Cao; Gökhan S. Hotamisligil and Alexander R. Ivanov (2007)
"Proteomic Analysis of Murine Adipose Tissue Using Pressure Cycling Technology and High Resolution Tandem Mass Spectrometry U.S. HUPO (2007)"
- Gary B. Smejkal, D. C. Muddiman, M. H. Schweitzer, T. S. Collier and A. T. Kwan (2007)
"Electrophoretic Analyses of Proteins and Peptides Isolated From Cortical Bone Using a Pressure Cycling Technology"
-Third Annual US HUPO Conference, Seattle WA.
Pressure BioSciences, Inc., South Easton, MA
Smejkal & Schweitzer ERP article published: "Will current technologies enable dinosaur proteomics?
Gary B. Smejkal, Mary H. Schweitzer
Future Drugs, Ltd. 2007
Solution phase isoelectric fractionation in the multicompartment electrolyser: A divide and conquer strategy for the analysis of complex proteomes
Gary B. Smejkal, Alexander Lazarev
BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. VOL 4. NO 1. 76–81. MAY 2005
Pressure Cycling Technology (PCT) Applications in Extraction of Biomolecules from Challenging Biological Sample
Feng Tao, Chunqin Li, Gary Smejkal, Alexander Lazarev, Nathan Lawrence, and Richard T. Schumacher
Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology , Vol. 1, 166-173, 2007
Proteomic Analyses of Proteins Isolated From Mammalian Bone
Gary B. Smejkal, Ada T. Kwan, Ilyana Romanovsky, David C. Muddiman, Mary H. Schweitzer, and Timothy S. Collier
HUPO 6th Annual World Congress Seoul, October 6 – 10, 2007
"Pressure Cycling Technology (PCT): a Novel Sample Preparation Approach to Biomarker Discovery and Drug Development in Lipid-Rich Samples"
Ilyana Romanovsky, Gary Smejkal, Alexander Lazarev, Nate Lawrence, and Richard T. Schumacher
Next Generation Pharmaceutical
Tris interference in IEF and 2-DE
Gary B. Smejkal, Myra H. Robinson
ELECTROPHORESIS May 10 2007, Volume 28, Issue 10, Pages: 1601-1606
Application of pressure cycling technology to tissue sample preparation for 2-DE
Heather Ringham, Richard L. Bell, Gary B. Smejkal, James Behnke, Frank A. Witzmann
ELECTROPHORESIS, March 6 2007, Volume 28, Issue 6, Pages: 1022-1024
- Extraction and Analysis of Genomic DNA from Ancient Bone Using Barocycler
- Analysis of RNA Recovery and Gene Expression in the Epidermis Using Non-invasive
Tape Stripping
Wong R, Tran V, Talwalker S, Benson NR.
2006 Nov;44(2):81-92. Epub 2006 Oct 2.
- An Analysis of Select Pathogenic Messages in Lesional and Non-lesional Psoriatic Skin
Using Non-invasive Tape Harvesting
Benson NR, Papenfuss J, Wong R, Motaal A, Tran V, Panko J, Krueger GG.
2006 Oct;126(10):2234-41. Epub 2006 Jun 1.
- Elucidation of the Frankia EAN1pec Genome Sequence Shows Gene Expansion
and Metabolic Versatility
Louis S Tisa, James M. Niemann, Tania Rawnsley, Teal Furnholm, David R Benson, Gary B. Smejkal, Pascal Lapierre, J.Peter Gogarten, Ying Huang, Juliana Mastronunzio, Carla A. Bassi, Philippe Normand, M. Pilar Francino, and Paul Richardson
U. New Hampshire, Durham, NH, USA; U Connecticut , Storrs, CT, USA; JGI, Walnut Creek, CA, USA, Pressure Biosciences, Inc, Bridgewater, MA, USA, niversité Lyon, Villeurbanne, France
- Exploration of the Actinorhizal Symbiosis: What can we learn from the Frankia genomes
and where do we go from here?
Louis S Tisa, David R Benson, Gary B. Smejkal, Pascal Lapierre, J. Peter Gogarten, Philippe Normand, M. Pilar Francino, and Paul Richardson
Dept. Microbiology , U New Hampshire, Durham, NH, USA; Dept. Mol. Cell Biol., U Connecticut , Storrs, CT, USA; JGI, Walnut Creek, CA, USA, Pressure Biosciences, Inc, Bridgewater, MA, USA, Ecologie Microbienne UMR CNRS 5557, Université Lyon, Villeurbanne, France
- Use of Pressure Cycling Technology (PCT) for the Release of DNA from Plants
Sandra Harrington, Susan McCouch , Feng Tao , Nate Lawrence , Richard T. Schumacher
Cornell University, 146 Emerson Hall, Ithaca, NY Boston Biomedica, Inc., 217 Perry Parkway, Gaithersburg, MD
- Novel Bacterial Protein Extraction Platform for Proteomic Analysis
Sun W. Tam, Douglas Hinerfeld and Jennifer D. Isbister
University of Massachusetts Medical School, Department of Biochemistry and Molecular Pharmacology, Proteomic Consortium
George Mason University, College of Science, Department of Molecular and Microbial Biosciences
- HTP Proteome-Glycome Analysis in Caenorhabditis Elegans
Heidi A. Geiser, Andy Hanneman, J. Cesar Rosa, Vernon Reinhold
Center for Structural Biology, Department of Chemistry, University of New Hampshire, Durham, NH; Universidade de Sao Paulo, Brazil
- Application of Pressure Cycling Technology (PCT) in Proteomics Increased Yield of
High Molecular Weight Proteins in Mouse Liver Lysates 02/09/06
Frank Witzmann, Heather Ringham, Gary Smejkal, James Behnke
Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA
Pressure BioSciences, Inc., West Bridgewater, MA, USA
-
Sample Preparation for Two-Dimensional Gel Electrophoresis using Pressure Cycling Technology
Gary B. Smejkal, Frank A. Witzmann, Heather Ringham, Deena Small, Susan F. Chase, James Behnke and Edmund Ting
Smejkal G.B. (2006). I'm an - Omics, You're an -Omics. Expert Rev. Proteomics, 3, 383-385.
- "Proteome Society Meeting".
Tao, F. and Smejkal, G.
Expert Rev. Proteomics, (2006), 3(1), 7-8
- "The promise of gel-based proteomics"
Smejkal G.B.
Expert Opinion in Drug Discovery, (2006), 1, 7-10.
- "Applications of Pressure Cycling Technology (PCT) in Proteomics"
Feng Tao, Behnke, J., Li, C., Saravis, C., Schumacher, R.T., and Lawrence, N.P.
Separation Methods in Proteomics, (2006), Smejkal, G.B. and Lazarev, A., Eds., CRC Press, Taylor & Francis, Baton Rouge, pp. 3 -18.
- "Simultaneous Reduction and Alkylation of Protein Disulfides in a Centrifugal Ultrafiltration Device Prior to Two-Dimensional Gel Electrophoresis"
Gary B. Smejkal, Li C., Robinson M.H., Lazarev, A.V., Lawrence, N.P., and Chernokalskaya, E.
J. Proteome Res., (2006), 5 (4), 983-987.
- "Increased Protein Yields from Escherichia coli Using Pressure Cycling Technology"
Gary B. Smejkal, Robinson M.H., Lawrence, N.P., Tao, F., Saravis, C.A., and Schumacher, R.T.
J. of Biomolecular Techniques, (2006), 17:173-175.
- G. B. Smejkal, F. Witzmann, H. Ringham, D. Small, E. Ting, I. Romanovsky, and A. Lazarev (2006).
"Applications of Pressure Cycling Technology (PCT) to Sample Preparation for Proteomic Analyses by Two-Dimensional Gel Electrophoresis"
-HUPO 5th International World Congress, Long Beach, CA
- Gary Smejkal, Witzmann, F., Ringham, H., Small, D., Chase, S., Thomas, W.K., Morris, K., Abebe, E., and Behnke, J. (2006).
"Applications of Pressure Cycling Technology (PCT) to Sample Preparation for Two-Dimensional Gel Electrophoresis".
- 7th Siena Meeting: From Genome to Proteome, Back to the Future. Siena, Italy. September 3-7, 2006.
- Feng Tao, C. Li, A. Lazarev, N. Lawrence, and R.T. Schumacher (2006)
"Extraction of Nucleic Acids from Challenging Biological Samples Using Pressure Cycling Technology"
- 2006 CHI, Nucleic Acid-Based Technologies-Meeting the Demands, Arlington, VA
- Gary Smejkal, D. Himali, A. Hanneman, and V. Reinhold (2006)
"Breaking the Tough Cuticle of Caenorhabditis elegans Using Pressure Cycling Technology and Optimized Lysis Reagents: Giving the Worms a Perm"
- 2006 US HUPO Second Annual Conference, Boston, MA
- Gary Smejkal, M. Robinson, A. Lazarev, C. Li, J. Behnke, F. Tao, R.T. Schumacher, and N. Lawrence (2006)
"Application of Pressure Cycling Technology (PCT) in Proteomics: Increased Cell Lysis and Protein Yields from Model Organisms"
- 2006 US HUPO Second Annual Conference, Boston, MA
- Gary Smejkal, A. Lazarev, F. Tao, N. Lawrence, and R.T. Schumacher (2006)
"A Comparison of Pressure Cycling Technology (PCT) to Other Methods for Protein Extraction"
- 2006 MASSEP Eighth Symposium, North Eastern University, Boston, MA
- Smejkal, G., F. Tao, N. Lawrence, and R.T. Schumacher (2006)
"A Comparison of Pressure Cycling Technology (PCT) to Other Methods of Protein Extraction"
- 2006 AACC, Oakridge Conference, San Jose, CA
- Biomarker Profiles of Echinacea Species Using Pressure Cycling Technology and
MALDI-TOF Mass Spectrometry
R.K Harris, L.A. Greene, S.A. Schwartz, J.R. Guthrie, and D.E. Gray,
Midwest Research Institute, Kansas City, MO 64110, USA
- Pressure Cycling Technology and Its Application in Steroid Receptor Extraction
D. Alan Kerr; Irina A. Smolenkova; Andrei S. Smolenkov; James L. Wittliff
Journal of Clinical Ligand Assay, Spring 2004, Volume 27, Number 1, pp. 18-23(6)
- Harrington, S., McCouch, S., Tao, F., Lawrence, N., Schumacher, R.T. (2004)
"Use of Pressure Cycling Technology (PCT) for the Release of DNA from Plants"
- Annual Plant, Animal and Microbes Genomics Conference, San Diego, CA
- Tao, F., Lawrence, N., Li, C., Behnke, J., Lathey, J., Manak, M., & Schumacher, R.T. (2004)
"Deciphering Mechanisms for Infectious Agent Inactivation Using Cyclic High Pressure or Pressure Cycling Technology (PCT)"
- Annual Biophysical Society Meeting, Baltimore, MD
- Lawrence, N., Tao, F., Kakita, A., Christian, T., Petersen, D., Behnke, J., Li, C., Saravis, C., Manak, M, & Schumacher, R.T. (2004)
"Introduction of a Bench Top Instrument Employing Pressure Cycling Technology (PCT) for the Extraction of Nucleic Acids and Proteins"
- PITTCON2004, Chicago, IL
- Tao, F., Feng, ZC, Lawrence, NP, & Schumacher, R.T. (2004)
"Deciphering the Mechanisms of Action in the Release of Molecules from Biological Samples Using Pressure cycling Technology (PCT)"
- Poster at 10th Annual FDA Science Forum, Washington, DC
- Lathey, J.L., Nakhai, B., Tuzmen, P., Tao, F., Schumacher, R.T., Manak, M.M, & Lawrence, N.P. (2004)
"Inactivation of HIV Inactivation of HIV-1 using Pressure Cycling Technology (PCT)"
- ASM Conference, New Orleans, LA
- Tao, F., Lawrence, N.P., Behnke, J., Li, C., Manak, M., Saravis, C. & Schumacher, R. (2004)
"Biological Sample Extraction Using Pressure Cycling Technology (PCT)"
- Poster at 6th National NIH SBIR Conference, Bethesda, MD
- Schumacher, R., Behnke, J., Li, C., Lawrence, N.P. & Tao, F. (2004)
"A New Benchtop System for Sample Preparation Using Pressure Cycling Technology (PCT)"
- Poster at the GSAC XVI, Washington, DC
- "Biological Sample Preparation System Using Pressure Cycling Technology (PCT)"
Tao, F, Lawrence, N.P., Miller, W.W., Li, C., Tuzmen, P., Behnke, J., Nakhai, B., Kakita, A., Christian, T., Reed, D. Manak, M.M., Schumacher, R.T.
Advances in High Pressure Bioscience and Biotechnology II, (2003), Springer, New York, p413-417
- "Tired of the Same Old Grind in the New Genomics and Proteomics Era?"
Garret, PE, Tao, F, Lawrence, N, Ji, J, Schumacher, RT, Manak, M
TARGETS, (2002), 1 (5): 156-162
- "Automated Solution for Sample Preparation: Nucleic Acid and Protein Extraction From Cells and Tissues Using Pressure Cycling Technology (PCT)"
Schumacher, RT, Manak, M, Garrett, P, Miller W, Lawrence, N, Tao, F
Am. Laboratory, (2002), 34(16): 38-43
- "Inactivation of viruses in plasma by cycled pulses of high pressure."
Dusing, S., Li, C., Behnke, J., Manak, M. and Schumacher, R.
Trends in High Pressure Bioscience and Biotechnology, (2001), R. Hayashi, ed. Elsevier Science B.V. 355-359
- "Pre-analytical sterilization of serum by pressure cycling treatment:
A novel procedure to prevent laboratory acquired infections."
Remaley, A., Hess, R., Fischer, S., Sampson, M. and Manak, M.
Clin Chem 46:, (2000), (S6) A39.
- "Pressure Cycling Technology: A Novel Approach to Viral Inactivation in Plasma."
Bradley, D.W., Hess, R.A., Tao, F., Sciaba-Lentz, L., Remaley, A.T., Laugharn, J. A., Manak, M.
Transfusion, (2000), 40, 193-200.
- "A bioseparation apparatus with high-pressure fluid injection and fluid sampling."
Dreier G.H., Tao F., Hess R.A., Cheung C.Y., Sciaba L.E., Green D.G., Laugharn J.A. Jr.
Analytical Biochemistry, (1999), 269(2), 223-9.
- "Unusual Properties of Highly Charged Buffers: Large Ionization Volumes
and Low Barrier Hydrogen Bonds."
Hess, R.A., Reinhard, L.A.
JACS, (1999), 121 (42), 9867-9870.
- "Sample preparation for molecular diagnostics by pressure cycling."
Laugharn J.A.Jr., Tao F., Sciaba-Lentz L., Bradley, D.W., Hess R.A.
High Pressure Bioscience & Biotechnology, (1998), Ludwig (Ed.), Springer, New York.
- "Use of High Pressure to Accelerate Antibody:Antigen Binding Kinetics Demonstrated in
and HIV-1 p24:Anti-HIV-1 p24 Assay."
Green, D.J, Litt, G.J., and Laugharn, J.A. Jr.
Clin. Chem., (1998), 44, 341-342.
- "High-pressure-mediated dissociation of immune complexes demonstrated in model systems."
Cheung, C.Y., Green, D.J., Litt, G.J. and Laugharn, J.A., Jr.
Clin. Chem., (1998), 44, 299-303.
- "Reversible inhibition of lambda exonuclease with high pressure."
Rudd, E.A.
Biochem. Biophys. Res. Commun, (1997), 230, 140-142.