Evaluation of Pressure Cycling Treatment on Barocycler’ NEP3229 for Extraction of Low-Template Forensic DNA Samples

This presentation will summarize our efforts to increase recovery of DNA from low-template samples though the use of a pressure cycle treatment (PCT) using the Barocycler® NEP3229 (Pressure BioSciences). Specific goals, including simultaneous incubation of samples, maximizing DNA yield, and evaluation of instrument parameters and consumables for the target sample, were addressed. An existing QIAGEN protocol was compared to a modified version incorporating pressure cycling pretreatment.