Abstract: The current study investigated synergism of elevated hydrostatic pressure, habituation, mildAbstract: The current study investigated synergism of elevated hydrostatic pressure, habituation, mildheat, and antimicrobials for inactivation of O157 and non-O157 serogroups of Shiga toxin-producingEscherichia coli. Various times at a pressure intensity level of 450 MPa were investigated at 4 and45 C with and without carvacrol, and caprylic acid before and after three-day aerobic habituationin blueberry juice. Experiments were conducted in three biologically independent repetitions eachconsist of two replications and were statistically analyzed as a randomized complete block designstudy using ANOVA followed by Tukey- and Dunnett’s-adjusted mean separations. Under thecondition of this experiment, habituation of the microbial pathogen played an influential (p < 0.05) roleon inactivation rate of the pathogen. As an example, O157 and non-O157 serogroups were reduced(p < 0.05) by 1.4 and 1.6 Log CFU/mL after a 450 MPa treatment at 4 C for seven min, respectively,before habituation. The corresponding log reductions (p < 0.05) after three-day aerobic habituationwere: 2.6, and 3.3, respectively at 4 C. Carvacrol and caprylic acid addition both augmented thepressure-based decontamination ecacy. As an example, Escherichia coli O157 were reduced (p < 0.05)by 2.6 and 4.2 log CFU/mL after a seven-