•  Overall, a 2.5-log reduction (P<0.05) i.e. >99% inactivation of the habituated 6-strainrifampicin resistant Escherichia coli O157:H7 was achieved through application ofelevated hydrostatic pressure at 4°C

.•  Overall, a 5-log reduction (P<0.05) i.e. 99.999% inactivation of the habituated 5-strain Salmonella serovars was achieved through application of elevated hydrostaticpressure at 25°C.

•  Hydrostatic pressure processing is able to inactivate both pathogens more than 99% at the applied time and pressure.