Forensics

Forensics

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Pressure Cycling Technology (PCT) Reduces Effects of Inhibitors of the PCR

Pamela L. Marshall1, Jonathan L. King1, Nathan P. Lawrence2, Alexander Lazarev2, Vera S. Gross2, Bruce Budowle1

1Institute of Applied Genetics, Department of Forensic and Investigative Genetics, UNT Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, USA 2Pressure BioSciences Incorporated, 14 Norfolk Avenue, South Easton, MA 02375, USA

Pressure Cycling Technology (PCT) Applications for DNA Extractions from Challenging Forensic Samples

Suzanne Gonzalez; Elizabeth Feller; Dixie Peters; Bruce Budowle; and Arthur Eisenberg

University of North Texas Health Science Center, Institute of Investigative Genetics, UNT Center for Human Identification, 3500 Camp Bowie Blvd., Fort Worth, TX 76107

Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology

Kathryn A. Harper1; Kelly A. Meiklejohn1; Richard T. Merritt2;  Jessica Walker3; Constance L. Fisher4; James M. Robertson5

 

1Counterterrorism and Forensic Science Research Unit, Visiting Scientist Program, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 2DNA Support Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 3Trace Evidence Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 4DNA Casework Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 5Counterterrorism and Forensic Science Research Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA

 

Improved DNA Profiles from Aged Horse Feces Using Pressure Cycling Technology

K. Deshpande1,2; M. Villarreal1,2; D. K. Mills1,2

1Department of Biological Sciences, Florida International University, OE 167, 11200 SW 8th Street, Miami, FL 33199, USA, 2International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA

Forensics

Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology.

Harper KA, Meiklejohn KA, Merritt RT, Walker J, Fisher CL, Robertson JM. SLAS Technol. 2018 Feb;23(1):97-105. doi: 10.1177/2472630317732073. Epub 2017 Oct 4.PMID: 28977757

Pressure-based alkaline lysis with immunocapture, a method for enhanced recovery in differential extraction.

Martinez V, Nori D, Dimsoski P, McCord B. Electrophoresis. 2017 Nov;38(21):2777-2785. doi: 10.1002/elps.201700139. Epub 2017 Aug 21.PMID: 28714088

The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.

Nori DV, McCord BR. Anal Bioanal Chem. 2015 Sep;407(23):6975-84. doi: 10.1007/s00216-015-8737-8. Epub 2015 Jun 9.PMID: 26055880

                                                                                                                     

Pressure cycling technology (PCT) reduces effects of inhibitors of the PCR.

Marshall PL, King JL, Lawrence NP, Lazarev A, Gross VS, Budowle B.

Int J Legal Med. 2013 Mar;127(2):321-33. doi: 10.1007/s00414-012-0770-y. Epub 2012 Sep 18.PMID: 22986474