•  The high pressure facilitates conformation changes of the target glycoprotein, increasing the accessibility of the endoglycosidase to the cleavage sites.
• 1:2500 enzyme : substrate molar ratio at 30 kPsi and 37^oC quantitatively released the asparagine linked glycans in minutes.
• Pressure cycling apparently did not lead to any loss of sialic acid residues.
• The microliter scale reaction volume alleviated possible precipitation related issues.
•  PCT offers simultaneous processing of 12 samples.