Pressure-Enhanced Enzymes Application Notes Display: ASC DESC Sort By Title Date Ordering Guidelines for Protocol Development: Introduction Pressure-enhanced proteolytic digestion exploits the ability of high hydrostatic pressure to promote protein denaturation and the access of proteolytic enzymes to their target sites. Pressure denaturation is fundamentally different from thermal denaturation as it occurs by virtue of hydration of hydrophobic residues and by water saturation of protein substrate cavities normally inaccessible to solvent. Pressure denaturation is more efficient for hydrophobic proteins, while some hydrophilic soluble proteins are reported to retain relatively compact conformation, even when saturated by water molecules [1-6]. Download View Details Proteolysis-PrEP: Optimization of Pressure Cycling Conditions for Efficient Digestion of Native IgG Introduction PCT has been proven to accelerate enzymatic protein digestion and the positive effect of PCT on digestion by both trypsin and Lys-C is well established [1-4]. Additionally, the enhancing effects of PCT on the activity of enzymes including Proteinase K, PNGase F, chymotrypsin, and lysozyme have been reported [5-8]. High hydrostatic pressure accelerates protein digestion via a combination of two mechanisms. Download View Details Proteolysis-PrEP: Activity and Specificity of Chymotrypsin Under Pressure Cycling Condition IntroductionPressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion is well established for improved sequence coverage, higher recovery and significantly reduced digestion times [1-5]. Additionally, the enhancing effects of PCT on the activity of other enzymes, including Proteinase K, PNGase F, Glu-C, Lys-C, and lysozyme have been reported [6-12]. Download View Details Proteolysis-PrEP: Pressure-accelerated Activity of Endoproteinase Glu-C IntroductionThe positive effect of Pressure Cycling Technology (PCT) on trypsin digestion is well established [1-6], and has been shown to result in improved sequence coverage, higher recovery and significantly reduced digestion times. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Lys-C, Proteinase K, PNGase F, chymotrypsin and lysozyme has been reported [7-13]. Download View Details Proteolysis-PrEP (Lysozyme): Effect of Pressure Cycling on Lysozyme Activity IntroductionPressure cycling technology (PCT) has been proven to accelerate enzymatic protein digestion. For example, the effect of PCT on trypsin digestion has been demonstrated by several laboratories. They report that digestion times can be reduced from hours to minutes [1, 2]. Not only has PCT been shown to accelerate and improve protein digestion in solution, but it also can accelerate the digestion by trypsin of proteins in polyacrylamide gel slices [3]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, and Lys-C, has been reported [4, 5, 6]. Download View Details Start Prev 1 2 3 4 Next End