Alexander Lazarev, Pressure BioSciences, Inc.
There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.
High Pressure Induced DNA Damage in Escherichia coli Involves Pressure-Mediated Dissociation of the Tetrameric Mrr Restriction Endonuclease
Anais Bourges1,2, Oscar E. Torres M.3, Anirban Ghosh3, Wubishet M. Tadesse3, Nathalie Declerck2, Abram Aertsen3 and Catherine Royer1 1 Department of Biological Sciences, Renssealer Polytechnic Institute, Troy NY, USA 2 Centre de Biochimie Structural, CNRS-Universite Montpellier UMR 5048, Montpellier, France 3 Laboratory of Food Microbiology, Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium
Tiannan Guo1; Li Li2; Ulrich Wagner3; Qing Zhong3; Christine Fritz3; Christian Fankhauser3; Cedric Poyet3; Rebecca Hartmann3; Andreas Beyer2; Peter Wild3; Ruedi Aebersold1, 4 1 Dep Biol, Inst Mol Sys Biol, ETH Zurich, Zurich, Switzerland 2 CECAD, University of Cologne, Cologne, Germany 3 Institute of Surgical Pathology, University Hospital, Zurich, Switzerland 4 Faculty of Science, University of Zurich, Zurich, Switzerland
From PCT-HD to SWATH� Data Acquisition in Half a Day
Vera Gross, Ph.D. >Senior Scientist
Deepthi Nori and Bruce McCord
Culture: Lyophilised Baker’s Yeast (Saccharomyces cerevisiae) was used to inoculate 200 mL sterile YMB (Yeast Mold Broth: Peptone 5 g/L, Dextrose 10 g/L, Maltose 3 g/L, Yeast Extract 3g/L) in 1 L flasks which were then incubated at 30°C with shaking at approximately 100 rpm for 24 hours. Disruption: The resulting culture was passed in 30 mL aliquots through either a Constant Systems Ltd. ‘Z+’ 1.1 kW Continuous Flow Cell Disruptor or a Thermo Spectronic French Pressure Cell Press at 40 kpsi. The samples were weighed before and after being passed through the machines, and the time taken to process was recorded. The machines were rinsed with 30 mL deionised water between each use. Counting: After being passed through the machines, 10 µL of lysate was mixed 1:1 with the viability stain Trypan Blue. White live cells and blue dead cells were counted using a hemocytometer. A sample of unlysed cells from the same culture was used as a control, from which the percentage partial lysis (including blue cells) and complete lysis was calculated.
Slides: PCT-HD for Tissue Biopsy Samples: Comparison to a Standard Method
Vera Gross1; Peter Hains3; Keith Ashman2; Val Valova3; Alexander Lazarev1 1 Pressure BioSciences, Inc., South Easton MA, USA 2 SCIEX, Framingham MA, USA 3 Children's Medical Research Institute, Westmead NSW, Australia
Anna Tomina; Alexander Lazarevb; Matthew P. Berea; Hana Redjeba; and Bela Torok* a a University of Massachusetts Boston, 100 morrissey Blvd., Boston, MA, USA. E-mail: email@example.com; Fax: (+1)-617-287-6030 b Pressure BioSciences Inc., 14 Norfolk Avenue, South Easton, MA, USA
The Best Approach: Homogenization or Manual Permeabilization of Human Skeletal Muscle Fibers for Respirometry?
Steen Larsena, Regitze Kraunsoea, Martin Grama, Erich Gnaigerb, Jorn W. Helgea, Flemming Delaa a Xlab, Center for Healthy Aging, Department of Biomedical Sciences, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark b Department of Visceral, Transplant, and Thoracic Surgery, D. Swarovski Research Laboratory, Medical University of Innsbruck, A-6020 Innsbruck
Hands Free Sample Homogenization and Protein Extraction from Small Tissue Biopsy Using Pressure Cycling Technology and MicroPestle
Shiying Shao1,2; Vera Gross3; Wen Yan1,2; Tiannan Guo2; Alexander Lazarev3; Ruedi Aebersold1,4 1 Institute of Molecular Systems Biology, ETH, Zurich, Switzerland 2 Huazhong University of Science & Technology, Wuhan, China 3 Pressure BioSciences, Inc., South Easton, MA 4 University of Zurich, Zurich, Switzerland