Pamela L. Marshall & Jonathan L. King & Nathan P. Lawrence & Alexander Lazarev & Vera S. Gross & Bruce Budowle
There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.
Structure-Relaxation Mechanism for the Response of T4 Lysozyme Cavity Mutants to Hydrostatic Pressure
Michael T. Lercha,b,1; Carlos J. Lopeza,b,1,2; Zhongyu Yangab; Margaux J. Kreitmana, b; Joseph Horwitza; and Wayne L. Hubbella,b,3 a: Jules Stein Eye Institute, University of California, Los Angeles, CA 90095 b: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095
Discovery of Mitochondrial Protein Biomarkers of Atrial Fibrillation Using Unique Human Tissue Samples
Maryam Goudarzi1, Mark M. Ross1, Weidong Zhou1, Amy Van Meter1, Emanuel Petricoin1, Lance Liotta1, Lisa Martin2, Chidima Martin2 and Niv Ad2 1 George Mason University, Manassas, VA 2 Inova Heart & Vascular Institute, Falls Church, VA
Ruedi Aebersold, Ph.D Institute of Molecular Systems Biology, ETH-Zurich
More Efficient Tissue Lysis and Protein Digestion with Lower Concentration of Denaturant Using Pressure Cycling Technology
Wen Yan1,2; Shiying Shao1,2; Vera Gross3; Alexander Lazarev3; Tiannan Guo1; Ruedi Aebersold1,4 1 Institute of Molecular Systems Biology, ETH, Zurich, Switzerland 2 Huazhong University of Science & Technology, Wuhan, China 3 Pressure BioSciences, Inc, South Easton, MA 4 University of Zurich, Zurich, Switzerland
Extraction of Proteins and RNA from Small Solid Tissue Samples Using PCT and FT 500-ND PULSE Tubes-080610
Introduction Efficient, reproducible and rapid tissue disruption and extraction of biomolecules are prerequisite for many biological applications. Solid tissues, especially tough or fibrous ones like muscle, generally require extensive mechanical disruption prior to extraction. Mortar and pestle grinding, pulverization in liquid nitrogen or homogenization with a dounce or polytron homogenizer are some of the classical methods that have been used for tissue disruption. However, these manual methods are often inconsistent, time consuming and potentially hazardous. In addition, due to the amount of sample loss inherent in these methods, they are often unsuitable for use with small samples. Here we describe a system for efficient tissue disruption and extraction of protein or RNA from solid tissues using the Pressure Cycling Technology Sample Preparation System (PCT SPS) and the FT 500-ND PULSE Tubes. Pressurization of small sample volumes in these tubes causes repeated compression of the sample tissue between the PULSE Tube Cap and Ram. This high-pressure mechanical tissue disruption, combined with the power of pressure cycling technology (PCT), is an efficient and reproducible method to prepare whole tissue lysates from solid tissue samples for extraction of proteins or nucleic acids.
Isolation of Mitochondria from Rat Lung Using The PCT Shredder and Pressure Cycling Technology (PCT)
Introduction: Isolation of intact mitochondria from human and animal tissue is crucial for studies in aging, diabetes and cancer. High quality functional mitochondrial isolates are also important for drug screening studies . Mitochondria isolation from solid tissue is usually performed by labor-intensive homogenizer-based methods  that require extensive operator experience. To facilitate efficient and reproducible mitochondria preparation, we have developed a semi-automated method to release mitochondria from solid fibrous tissues, such as lung, using The PCT Shredder for tissue homogenization. This method can be combined with a subsequent treatment using pressure cycling technology (PCT) to further increase yield, if desired.
Alexander Lazarev, Pressure BioSciences, Inc.
Comparison Between Ultra-High Pressure and Atmospheric Tryptic Tigestion for Proteomic Analyses
E. Bonneil1, R. Biringer2, C. Bell1, P. Thibault1 1 Institute for Research in Immunology and Cancer Universite de Montreal 2 Thermo Fisher Scientific
1 Facility Center California R.A. Eigenheer1, J.T. Smilowitz2 and B.S. Phinney1 1. Proteomics Core Facility, Genome Center, University of California, Davis 2. Department of Food Science and Technology, University of California, Davis