Daeho Shin1, Han Sol Kim2, Jungbae Kim2 1BMTech Medial Co. Ltd., Seoul, Korea 2Chemical and Biological Engineering, Korea University, Seoul, Korea Abstract Only
There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.
14 Norfolk Ave., South Easton, MA 02375
K. Deshpande1,2, M. Villarreal1,2, D. K. Mills1,2 1 Department of Biological Sciences, Florida International University, OE 167, 11200 SW 8th Street, Miami, FL 33199, USA 2 International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA
Various Constant Systems Publications and Application
Constant Systems Cell Disruptors are highly utilised by research groups in the areas of drug discovery, protein characterisation and functional studies of cellular processes. To date, CS cell disruptors have directly contributed to research published in over 1,200 peer reviewed papers across a range of academic journals, including Nature and the Journal of Biological Chemistry.
Model HUB440 and HUB880 Advanced High Pressure Generators Fields of Use
Booth Poster: Barocycler 2320EXT Ideal for PCT-SWATH
The Barocycler 2320EXT Pressure Range: ambient - 45,000psi (310 Mpa) Max Temperature: 95°C Min Temperature: 4°C with circulating waterbath, ambient with electric heating Insulated closure for use during high temperature runs Process up to 16 samples simultaneously Easily accessible USB port on front panel Choice of temperature control - heating/cooling or heating only Requires 110psi input air pressure Computer-operated control with Touch Screen Interface Pressure medium: Distilled Water
SWATH For Quantitative Proteomics
Randy Arnold, PhD – SCIEX Pressure Biosciences ASMS Breakfast Seminar, June 6, 2017
Proteolysis-PrEP: Optimization of Pressure Cycling Conditions for Protein Digestion with Trypsin in Solution
Introduction Pressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion has been demonstrated by several laboratories [1, 2, 3] who have reported improved sequence coverage, higher recovery and significantly reduced digestion times when the trypsin reactions were carried out under pressure. Not only has PCT been shown to accelerate and improve digestion in solution, but it can also accelerate in-gel trypsin digestion [4, 5]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, Lys-C and lysozyme has been reported [6, 7, 8, 9].