There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.

Various Constant Systems Publications and Application

Constant Systems Cell Disruptors are highly utilised by research groups in the areas of drug discovery, protein characterisation and functional studies of cellular processes. To date, CS cell disruptors have directly contributed to research published in over 1,200 peer reviewed papers across a range of academic journals, including Nature and the Journal of Biological Chemistry.

Model HUB440 and HUB880 Advanced High Pressure Generators Fields of Use

Pressure BioSciences

Booth Poster: Barocycler 2320EXT Ideal for PCT-SWATH

The Barocycler 2320EXT Pressure Range: ambient - 45,000psi (310 Mpa) Max Temperature: 95°C Min Temperature: 4°C with circulating waterbath, ambient with electric heating Insulated closure for use during high temperature runs Process up to 16 samples simultaneously Easily accessible USB port on front panel Choice of temperature control - heating/cooling or heating only Requires 110psi input air pressure Computer-operated control with Touch Screen Interface Pressure medium: Distilled Water

SWATH For Quantitative Proteomics

Randy Arnold, PhD – SCIEX Pressure Biosciences ASMS Breakfast Seminar, June 6, 2017

Proteolysis-PrEP: Optimization of Pressure Cycling Conditions for Protein Digestion with Trypsin in Solution

Introduction Pressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion has been demonstrated by several laboratories [1, 2, 3] who have reported improved sequence coverage, higher recovery and significantly reduced digestion times when the trypsin reactions were carried out under pressure. Not only has PCT been shown to accelerate and improve digestion in solution, but it can also accelerate in-gel trypsin digestion [4, 5]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, Lys-C and lysozyme has been reported [6, 7, 8, 9].