Biophysics

Biophysics

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The consequences of cavity creation on the folding landscape of a repeat protein depend upon context

Kelly A. Jenkins, Martin J. Fossat, Siwen Zhang, Durgesh K. Rai, Sean Klein, Richard Gillilan, Zackary White, Grayson Gerlich, Scott A. McCallum, Roland Winter, Sol M. Gruner, Doug Barrick, Catherine A. Royer

Proc Natl Acad Sci U S A. 2018 Aug 28; 115(35): E8153–E8161. Published online 2018 Aug 13. doi: 10.1073/pnas.1807379115

PMCID: PMC6126725

Structure-relaxation mechanism for the response of T4 lysozyme cavity mutants to hydrostatic pressure

Michael T. Lerch, Carlos J. López, Zhongyu Yang, Margaux J. Kreitman, Joseph Horwitz, Wayne L. Hubbell

Proc Natl Acad Sci U S A. 2015 May 12; 112(19): E2437–E2446. Published online 2015 Apr 27. doi: 10.1073/pnas.1506505112 PMCID: PMC4434698

High-pressure small-angle X-ray scattering cell for biological solutions and soft materials

Durgesh K. Rai, Richard E. Gillilan, Qingqiu Huang, Robert Miller, Edmund Ting, Alexander Lazarev, Mark W. Tate, Sol M. Gruner

J Appl Crystallogr. 2021 Feb 1; 54(Pt 1): 111–122. Published online 2021 Feb 1. doi: 10.1107/S1600576720014752 PMCID: PMC7941318

High-pressure small-angle X-ray scattering cell for biological solutions and soft materials.

Rai DK, Gillilan RE, Huang Q, Miller R, Ting E, Lazarev A, Tate MW, Gruner SM.

J Appl Crystallogr. 2021 Feb 1;54(Pt 1):111-122. doi: 10.1107/S1600576720014752. eCollection 2021 Feb 1.PMID: 33841059

Viewing rare conformations of the β2 adrenergic receptor with pressure-resolved DEER spectroscopy

Michael T. Lerch, Rachel A. Matt, Matthieu Masureel, Matthias Elgeti, Kaavya Krishna Kumar, Daniel Hilger, Bryon Foys, Brian K. Kobilka, Wayne L. Hubbell

Proc Natl Acad Sci U S A. 2020 Dec 15; 117(50): 31824–31831. Published online 2020 Nov 30. doi: 10.1073/pnas.2013904117 PMCID: PMC7749303

An oligomeric switch controls the Mrr-induced SOS response in E. coli.

Bourges AC, Torres Montaguth OE, Tadesse W, Labesse G, Aertsen A, Royer CA, Declerck N.DNA Repair (Amst). 2021 Jan;97:103009. doi: 10.1016/j.dnarep.2020.103009. Epub 2020 Nov 6.PMID: 33220536

Pressure dependence of side chain 1H and 15N-chemical shifts in the model peptides Ac-Gly-Gly-Xxx-Ala-NH2

Markus Beck Erlach, Joerg Koehler, Claudia E. Munte, Werner Kremer, Edson Crusca, Jr, Masatsune Kainosho, Hans Robert Kalbitzer

J Biomol NMR. 2020; 74(8): 381–399. Published online 2020 Jun 22. doi: 10.1007/s10858-020-00326-w

PMCID: PMC7508751

Pressure dependence of side chain 13C chemical shifts in model peptides Ac-Gly-Gly-Xxx-Ala-NH2.

Beck Erlach M, Koehler J, Crusca E Jr, Munte CE, Kainosho M, Kremer W, Kalbitzer HR.J Biomol NMR. 2017 Oct;69(2):53-67. doi: 10.1007/s10858-017-0134-5. Epub 2017 Sep 14.PMID: 28913741

NMR derived changes of lipoprotein particle concentrations related to impaired fasting glucose, impaired glucose tolerance, or manifest type 2 diabetes mellitus.

Kalbitzer T, Lobenhofer K, Martin S, Beck Erlach M, Kremer W, Kalbitzer HR.Lipids Health Dis. 2023 Mar 24;22(1):42. doi: 10.1186/s12944-023-01801-7.PMID: 36964528


The pressure and temperature perturbation approach reveals a whole variety of conformational substates of amyloidogenic hIAPP monitored by 2D NMR spectroscopy.

Beck Erlach M, Kalbitzer HR, Winter R, Kremer W.Biophys Chem. 2019 Nov;254:106239. doi: 10.1016/j.bpc.2019.106239. Epub 2019 Jul 30.PMID: 31442763

Mapping protein conformational heterogeneity under pressure with site-directed spin labeling and double electron–electron resonance

Michael T. Lerch, Zhongyu Yang, Evan K. Brooks, Wayne L. Hubbell

Proc Natl Acad Sci U S A. 2014 Apr 1; 111(13): E1201–E1210. Published online 2014 Mar 18. doi: 10.1073/pnas.1403179111 PMCID: PMC3977274

Practical aspects of high-pressure NMR spectroscopy and its applications in protein biophysics and structural biology

José A. Caro, A. Joshua Wand

Methods. Author manuscript; available in PMC 2019 Sep 15.

Published in final edited form as: Methods. 2018 Sep 15; 148: 67–80. Published online 2018 Jun 30. doi: 10.1016/j.ymeth.2018.06.012 PMCID: PMC6133745

High pressure activation of the Mrr restriction endonuclease in Escherichia coli involves tetramer dissociation.

Bourges AC, Torres Montaguth OE, Ghosh A, Tadesse WM, Declerck N, Aertsen A, Royer CA. Nucleic Acids Res. 2017 May 19;45(9):5323-5332. doi: 10.1093/nar/gkx192.PMID: 28369499

Quantitative High-Resolution Imaging of Live Microbial Cells at High Hydrostatic Pressure.

Bourges AC, Lazarev A, Declerck N, Rogers KL, Royer CA.Biophys J. 2020 Jun 2;118(11):2670-2679. doi: 10.1016/j.bpj.2020.04.017. Epub 2020 Apr 23.PMID: 32402241

High-pressure EPR spectroscopy studies of the E. coli lipopolysaccharide transport proteins LptA and LptC.

Schultz KM, Klug CS.Appl Magn Reson. 2017 Dec;48(11-12):1341-1353. doi: 10.1007/s00723-017-0948-z. Epub 2017 Sep 21.PMID: 29332998