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Pressure Cycling Technology (PCT) Reduces Effects of Inhibitors of the PCR

Pam Marshall, PhD Candidate

Mid America Forensic DNA Conference, April, 2013

Pressure Cycling Technology (PCT) Reduces Effects of Inhibitors of the PCR - Manuscript

Pamela L. Marshall; Jonathan L. King; Nathan P. Lawrence; Alexander Lazarev; Vera S. Gross; Bruce Budowle

Improved DNA Profiles from Aged Horse Feces Using Pressure Cycling Technology

K. Deshpande1,2; M. Villarreal1,2; D. K. Mills1,2

1Department of Biological Sciences, Florida International University, OE 167, 11200 SW 8th Street, Miami, FL 33199, USA, 2International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA

Differential Extraction Using Alkaline Lysis and Pressure

Deepthi Nori and Bruce McCord


The Application of Alkaline Lysis and Pressure Cycling Technology in the Differential Extraction of DNA from Sperm and Epithelial Cells Recovered from Cotton Swabs

Deepthi V. Nori1 & Bruce R. McCord1

1Department of Chemistry CP304, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA This email address is being protected from spambots. You need JavaScript enabled to view it.

Super Absorbent Lower-Layers of Sanitary Pads: A Challenge in Sexual Aggression Cases

Inês Gregório1,2 and Pilar Martínez1


1National Institute of Toxicology & Forensic Science (INTCF), José Echegaray 4, 28232 Las Rozas-Madrid, Spain, 2Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, University Research Institute for Police Sciences (IUICP) Ctra. Madrid-Barcelona km 33.6, 28871 Alcalá de Henares-Madrid, Spain


This email address is being protected from spambots. You need JavaScript enabled to view it.
This email address is being protected from spambots. You need JavaScript enabled to view it.

Pressure Cycling Technology (PCT) Applications for DNA Extractions from Challenging Forensic Samples

Suzanne Gonzalez; Elizabeth Feller; Dixie Peters; Bruce Budowle; and Arthur Eisenberg

University of North Texas Health Science Center, Institute of Investigative Genetics, UNT Center for Human Identification, 3500 Camp Bowie Blvd., Fort Worth, TX 76107

Evaluation of Pressure Cycling Treatment on Barocycler® NEP3229 for Extraction of Low-Template Forensic DNA Samples

Mary Thompson, MS, F-ABC; Rebecca Schultheiss Mikulasovich, MS; Mark Powell, MS, F-ABC; Michael Donley, MS, F-ABC; Roger Kahn, PhD, F-ABC

Harris County Institute of Forensic Sciences 1885 Old Spanish Trail Houston, TX 77054, USA

Pressure Cycling Technology (PCT) Reduces Effects of Inhibitors of the PCR

Pamela L. Marshall1, Jonathan L. King1, Nathan P. Lawrence2, Alexander Lazarev2, Vera S. Gross2, Bruce Budowle1

1Institute of Applied Genetics, Department of Forensic and Investigative Genetics, UNT Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, USA 2Pressure BioSciences Incorporated, 14 Norfolk Avenue, South Easton, MA 02375, USA

Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology

Kathryn A. Harper1; Kelly A. Meiklejohn1; Richard T. Merritt2;  Jessica Walker3; Constance L. Fisher4; James M. Robertson5

 

1Counterterrorism and Forensic Science Research Unit, Visiting Scientist Program, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 2DNA Support Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 3Trace Evidence Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 4DNA Casework Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA, 5Counterterrorism and Forensic Science Research Unit, Federal Bureau of Investigation Laboratory Division, Quantico, VA, USA

 

Pressure-Based Alkaline Lysis with Immunocapture, a Method for Enhanced Recovery in Differential Extraction

Vanesa Marinez; Deepthi Nori; Pero Dimimsoski; and Bruce McCord

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA

Forensics

Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology.

Harper KA, Meiklejohn KA, Merritt RT, Walker J, Fisher CL, Robertson JM. SLAS Technol. 2018 Feb;23(1):97-105. doi: 10.1177/2472630317732073. Epub 2017 Oct 4.PMID: 28977757

Pressure-based alkaline lysis with immunocapture, a method for enhanced recovery in differential extraction.

Martinez V, Nori D, Dimsoski P, McCord B. Electrophoresis. 2017 Nov;38(21):2777-2785. doi: 10.1002/elps.201700139. Epub 2017 Aug 21.PMID: 28714088

The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.

Nori DV, McCord BR. Anal Bioanal Chem. 2015 Sep;407(23):6975-84. doi: 10.1007/s00216-015-8737-8. Epub 2015 Jun 9.PMID: 26055880

                                                                                                                     

Pressure cycling technology (PCT) reduces effects of inhibitors of the PCR.

Marshall PL, King JL, Lawrence NP, Lazarev A, Gross VS, Budowle B.

Int J Legal Med. 2013 Mar;127(2):321-33. doi: 10.1007/s00414-012-0770-y. Epub 2012 Sep 18.PMID: 22986474