Model HUB440 and HUB880 Advanced High Pressure Generators Current User Applications
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Dr. Wayne Hubbell UCLA Jules Stein Eye Institute SDSL EPR - Protein Dynamics |
Molecular structure of a protein and the conformational changes that control its function are key to better drug design and development. Response to Pressure May Have Profound Implications in Future Drug Design & Development. |
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Dr. Cathy Royer Rensselaer Polytechnic Institute Protein Dynamics, Fluorescence, Two-photon Fluorescence Fluctuation Microscopy, In vivo High Pressure Biophysics
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70% of prokaryotes live under pressure in the bottom of the ocean. We study how proteins fold under pressure and how pressure effects live bacterial cells, in particular pressure-dependent induction of the SOS response in E.coli via the restriction endonuclease Mrr. The HUB has been a great system for us, as pressure changes can be programmed in advance and automatically implemented. In protein folding kinetic studies, the protein can be placed in the pressure cell and spectra can be acquired at increasing pressure levels without further input or attention from the user. Pressure changes are nearly instantaneous which is vital to our studies.
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Dr. Hans-Robert Kalbitzer University of Regensburg NMR: Protein and Membrane Dynamics
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We employ high pressure perturbation as a method of choice to detect excited conformational states of proteins. HUB440 pressure generator interfaced with Bruker NMR spectrometer allows us to perform a series of unattended high pressure NMR experiments overnight or the weekends, while spending our time on data analysis and writing manuscripts. The pressure generator is in regular use in our lab for more than four years.
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Dr. Martin Gruebele University of Illinois, Urbana-Champaign Fluorescence, In vivo High Pressure Biophysics
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How quickly can bacteria evolve pressure resistance? We combine the HUB and high-throughput sequencing to look for phenotypic and genetic changes in bacterial cells subject to cycles of high pressure. Our next application: looking at pressure denaturation of repeat proteins to see the interplay of folding and aggregation in action.
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Dr. Aliyar Fouladkah Tennessee State University Inactivation of Food pathogens
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With recent advancements in the engineering of machines, and consumer acceptability of pressure-treated products, this technology is gaining rapid adoption across many sectors of the food industry.
Members of my laboratory had a very positive experience using PBI high pressure bench-top system for obtaining accurate and precise data for inactivation of pathogens of public health concern.
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