Membrane proteins remain a significant unmet proteomics challenge
• Surfactant Extraction incompatible with
– ELISA or Ab arrays
– Chromatography (SEC, RPLC, or Electrophoresis)
– Mass Spectrometry
• In Sample Proteolysis
– Disconnects protein domains (intra-, extra-, and trans-membrane)
– Can’t study trans-membrane domains
v ProteoSolve™-TD Buffers with PCT allows
• recovery of membrane proteins intact
• proteins are stable in the extract (can be frozen and thawed)
• compatible with common proteomic methods:
– immunoaffinity capture (ELISA, Ab arrays, immunoaffinity chromatography)
– SDS-PAGE and 2-D gels.
– mass spectrometric analysis (post-affinity capture)
• simple one step extraction procedure
– ProteoSolve™-TD series buffers and PULSE tubes
– Pressure Cycling Technology (Pressure Biosciences)