There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.

Pressure induced SOS response in Escherichia coli involves Mrr, a Restriction Endonuclease

Anais Bourges1,2, Oscar E. Torres M.3, Anirban Ghosh3, Wubishet M. Tadesse3, Gilles Labesse2, Nathalie Declerck2, Abram Aertsen3 and Catherine Royer1 1 Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA 2 Centre de Biochimie Structural, CNRS-Université Montpellier UMR 5048, 34000 Montpellier, France 3 Department of Microbial and Molecular Systems, Laboratory of Food Microbiology, KU Leuven, B-3001 Leuven, Belgium

Streamlined Hands-Free Sample Preparation Using Pressure Cycling and PCT Pestle Sample Containers for Tissue Biopsy Profiling by SWATH-MS

Vera Gross1; Keith Ashman2; Peter Hains3; Alexander Lazarev*,1; Chris Hodgkins2; Phil Robinson3 1 Pressure BioSciences, Inc., 14 Norfolk Ave., South Easton, MA 02375, United States 2 SCIEX, 500 Old Connecticut Path Framingham, MA 01701, United States 3 Children's Medical Research Institute, 214 Hawkesbury Rd., Westmead NSW 2145, Australia

Poster: PCT-HD for Tissue Biopsy Samples: Comparison to a Standard Method

Discovery Starts With Sample Preparation-ASMS 2017

Dr. Alexander Lazarev, V.P. R&D Pressurebiosciences

Pressure-Accelerated Protein Digestion with Endoproteinase Lys-C

Introduction: Pressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion is well established [1-4] for improved sequence coverage, higher recovery and significantly reduced digestion times. Not only has PCT been shown to accelerate and improve digestion in solution, but it can also accelerate in-gel trypsin digestion [4, 5]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, chymotrypsin and lysozyme has been reported [6-10].