There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.

Application Note-PCT-HD: Homogenization, Extraction and Digestion of Small Tissue Samples for Proteomic Applications

The PCT-HD system is a combination of two of PBI’s innovative sample preparation tools; the NEP2320-Enhanced Barocycler and PCT MicroPestles. This unit can generate a maximum pressure of 45 kpsi (the original NEP2320 Barocycler is rated to 35 kpsi) and can be heated to temperatures up to 95°C. The higher pressure and temperature range give the NEP2320-Enhanced broad applicability in proteomics, enzymology and other fields of research.

Analysis of Tissue Biopsies by PCT-SWATH

Tiannan Guo, MD, PhD, Aebersold Lab, IMSB, ETH Zurich Presented at International HUPO 2013 Subset of Slides Please contact Dr. Tiannan Guo (guo@imsb.biol.ethz.ch) for more information.

The Proteome of Rhodopseudomonas palustris Revealed by Pressure Cycling Technology (PCT) and Two-Dimensional Gel Electrophoresis - 03/02/06

Rhodopseudomonas palustris is a Gram negative, purple, non-sulfur, phototropic bacterium, and is a metabolically versatile microbe. The bacterium can grow in the presence or absence of oxygen. In response to environmental changes, it can engage in alternative metabolic processes for cellular respiration. R. palustris can degrade the aromatic compounds comprising lignin, the second most abundant natural polymer. As such, it is being investigated for its potential in the removal of environmental pollutants [1]. The genome of R. palustris has been sequenced and annotated [2]. It follows that the analysis of this microorganism’s proteome has become an active area of research. Reliable proteomic analysis is contingent on the efficiency by which cells are lysed and their protein constituents released. Standard technique to efficiently lyse Gram-negative bacteria requires mechanical disruption of the cell, and either enzymatic or chemical breakdown of the cell wall.

Isolation of Functional Mitochondria from Whole Rat Brain Using a PBI Shredder and Pressure Cycling Technology (PCT)

Introduction Isolation of intact mitochondria from human and animal tissue is crucial for studies that focus on the elucidation of their function and dysfunction in conditions such as aging, diabetes and cancer. As potential drug targets, high quality functional mitochondrial isolates are important for drug screening studies [1]. Mitochondria isolation from solid tissue is usually carried out using labor-intensive homogenizer-based methods [2] that require extensive operator experience. Here we describe a semi-automated method to release mitochondria from solid rat brain tissue, using a PBI Shredder and PCT in place of traditional manual homogenization.

Improved Extraction of DNA of Ca. Liberibacter Species from Plants and Cultivated Cells Using Pressure Cycling Technology

A. Sechler1, A Marques2, N. Lawrence3, N. W. Schaad1 1FDWSRU, USDA-ARS, Fort Detrick, MD USA 2EMBRAPA, Brasilia, Brasil 3Pressure BioSciences Inc., South Easton, MA, USA