There are over 100 scientific papers published on the advantages of the PCT platform, many by key opinion leaders worldwide. Such advantages include: (a) extraction and recovery of more membrane proteins, (b) enhanced protein digestion, (c) differential lysis in a mixed sample base, (d) pathogen inactivation, (e) increased DNA detection and (f) exquisite sample preparation process control.
Johannes Burtschera, Luca Zangrandia, Christoph Schwarzera, Erich Gnaigerb,c
a Dept. Pharmacology, Medical University Innsbruck, Peter-Mayr-Str. 1a, 6020 Innsbruck, Austria
bD. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, Anichstr. 35, 6020 Innsbruck, Austria
cOROBOROS INSTRUMENTS, Schapfstr. 18, 6020 Innsbruck, Austria
Isolation of intact mitochondria from human and animal tissue is crucial for studies that focus on the elucidation of their function and dysfunction in conditions such as aging, diabetes and cancer. As potential drug targets, high quality functional mitochondrial isolates are important for drug screening studies . Mitochondria isolation from solid tissue is usually carried out using labor-intensive homogenizer-based methods  that require extensive operator experience. Here we describe a semi-automated method to release mitochondria from whole rat heart tissue, using a PBI Shredder and PCT in place of traditional manual homogenization.
Scott R. Kronewitter, Gordon W. Slysz, Daniel Lopez-Ferrer, Matthew E. Monroe, Rui Zhao, Thomas L. Fillmore, Karl K. Weitz, Robert A. Heegel, Daniel J. Orton, Brian L. LaMarche, Kevin L. Crowell, Anuj R. Shah, Carrie D. Nicora, Heather M. Brewer, David G. Camp II, Gordon A. Anderson, Ronald J. Moore, and Richard D. Smith
Extraction of proteins from extensively calcified osseous tissue, such as cortical bone has been particularly challenging for traditional methods of sample preparation. However, a comprehensive proteomic analysis of bone is only possible when the total protein constituency is effectively isolated. The efficiency of sample preparation is therefore a critical component of the analytical process. Historically, extraction of protein from bone required prolonged acid demineralization over several days to enable complete penetration of histochemical reagents to cellular components. Here we describe a method for the extraction of protein from ostrich tibia, which was used as a model sample to develop an extraction process that uses pressure cycling technology (PCT) and also which obviates the need for acid demineralization prior to extraction. The ability to extract proteins from bone without prior demineralization offers important advantages in efficient representative extraction of protein and significant time savings during sample preparation.